箭筈豌豆镉胁迫下的失水胁迫和渗透调节物质的积累  

Water Deficit Stress and Osmotic Substances Accumulation of Vicia sativa L. under Cadmium Stress

作　　者：芮海云[1,2] 张兴兴[2] 沈振国[2] 张芬琴[3] 

机构地区：[1]泰州学院医药与化学化工学院,江苏泰州225300 [2]南京农业大学生命科学学院,江苏南京210095 [3]河西学院农业与生物技术学院,甘肃张掖734000

出　　处：《作物杂志》2017年第3期69-74,共6页Crops

基　　金：国家自然科学基金(31160053);泰州学院博士基金(TZXY2015JBJJ005)

摘　　要：通过溶液培养试验,研究了2个箭筈豌豆(Vicia sativa L.)品种(镉耐性品种L3,镉敏感品种ZM)镉胁迫下含水量和渗透调节物质含量的变化,以及2个品种对失水胁迫的耐性。结果表明:25μmol/L镉处理14d,L3和ZM根中含水量分别较对照降低1.27%和4.66%,地上部含水量分别较对照降低4.06%和5.46%,ZM失水更明显;镉胁迫下,ZM和L3根中总氨基酸、脯氨酸和还原糖含量以及茎中总氨基酸含量都较对照显著升高,ZM较L3更明显;ZM叶中脯氨酸和还原糖含量也较对照显著升高,L3叶中二者较对照升高不显著;同时,ZM较L3有更强的对失水胁迫的耐性。因此,镉胁迫下箭筈豌豆渗透调节物质的积累是对镉胁迫引起的失水胁迫的积极响应;箭筈豌豆对失水胁迫的耐性不是决定其镉耐性的主要方面。We investigated the effects of cadmium stress on water content and osmotic substances accumulation in two Vicia sativa varieties （cadmium-tolerant variety L3, cadmium-sensitive variety ZM）, using solution culture experiments, and studied the tolerance of two varieties to water deficit stress. The results showed that water content decreased 1.27% and 4.66% in the roots, and 4.06% and 5.46% in the overground part of L3 and ZM, respectively. Content of total amino acid, proline and reducing sugar increased in the roots by two varieties and content of total amino acid increased in the stems by two varieties under cadmium stress, and the changes were more significant in ZM than in L3; Content of proline and reducing sugar increased significantly in the leaf of ZM but not in the leaf of L3. Meanwhile, Cd-sensitive variety ZM was more tolerant to water deficit stress than Cd-tolerant variety L3. The results indicated that accumulation of osmotic substances in V. sativa was a positive response to water deficit caused by cadmium stress. The tolerance to water deficit stress was not the main factor influencing the cadmium tolerance of V. sativa.

关 键 词：镉 箭筈豌豆 失水胁迫 渗透调节 

分 类 号：S551.9[农业科学—作物学]