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作 者:喻阑清 邹晓[1] 张健[1] 韩燕峰[1] 梁宗琦[1]
机构地区:[1]贵州大学生命科学学院真菌资源研究所,贵州贵阳550025
出 处:《微生物学通报》2017年第6期1370-1376,共7页Microbiology China
基 金:国家自然科学基金项目(No.31360031;31460010);国家自然科学基金重大项目子项目(No.31093440;31493010;31493011);贵州省农业科技攻关项目(黔科合NY[2013]3034)~~
摘 要:【目的】对寄生鞘翅目幼虫蛴螬的虫生真菌标本GZUIFR-lgs-1进行描述和鉴定。【方法】基于形态特征比较结合系统发育分析进行鉴定。【结果】该种通过形态特征产孢细胞单生,透明,多数与营养菌丝成近直角,基部具疣且明显膨大(19-27)μm×(2.7-3.6)μm,颈部长(12.0-14.5)μm,顶端呈螺旋状扭曲,偶有再育;分生孢子透明、光滑,橘瓣状,单生或偶有双生,外具黏液,大小为(4.8-6.0)μm×(2.4-3.6)μm,而与被毛孢属已知种相区别。基于ITS位点的系统发育分析支持形态学鉴定的结果。【结论】综合形态特征比较及系统发育分析结果,该种为被毛孢属的一个新的分类单元,命名为雷公山被毛孢,Hirsutella leigongshanensis。[Objective] A new entomogenous fungi (GZUIFR-lgs-1), which was parasitic on grub larvae was identified and described. [Methods] The analysis mainly based on phenotypic characteristics and molecular phylogeny. [Results] The strain distinguished from the other Hirsutella species by phialides solitary, hyaline, occasionally proliferation and near right angle to hyphae, with distinctly swollen and verrucose base (19-27) μm ×(2.7-3.6) μm, twisting in helices at the apex, neck (12.0-14.5) μm long; Conidia hyaline and smooth in mucous sheath, like orange segments (4.8-6.0) μm ×(2.4-3.6) μm, single or occasionally in pairs. The result of phylogenic analysis based on ITS loci supported the morphological identification. [Conclusion] The specimen GZUIFR-lgs-1 is a new species in the genus Hirsutella, supported by the phenotypic traits and molecular analysis, named Hirsutella leigongshanensis.
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