巴斯德毕赤酵母表达重组人对氧磷酶1的发酵条件优化  

Optimization of the Fermentation Conditions of Recombinant Human Paraoxonase 1 Expression in Pichia pastoris

作　　者：廖一波[1] 于子桐[1] 潘力[1,2] 叶燕锐[1,2] 

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]广东省发酵与酶工程重点实验室,广东广州510006

出　　处：《食品与发酵科技》2017年第3期1-7,共7页Food and Fermentation Science & Technology

基　　金：基金项目:华南理工大学中央高校基本科研业务费(2014ZM0056)

摘　　要：本研究采用响应面方法优化重组人对氧磷酶1(Recombinant Human Paraoxonase 1,rhPON1)在毕赤酵母中的表达。首先通过研究甲醇浓度、p H、培养基装液量3个单因素对rhPON1表达的影响,然后以rhPON1单位体积酶活力为响应值进行响应面分析。结果显示:甲醇浓度1.42%,p H值5.91,培养基装液量9.39%是最优发酵条件。在此优化条件下,rhPON1的单位体积酶活力预测值为0.737U/mL,与验证值(0.735U/mL)基本一致。人对氧磷酶1可以水解多种有机磷化合物,被认为是一种有机磷农药中毒的解毒剂。In this study, the expression of Recombinant Human Paraoxonase 1 （rhPON1） in Pichia pastoris was optimized by response surface method. A single-factor test coupled with response surface methodology （RSM） was used to adjust the fermentation conditions. The results showed that the optimized induction conditions were as fol- lows： methanol concentration of 1.42%, pH 5.91, culture volume of 9.39%, and the predicted theoretical enzyme activity of rhPON1 was 0.737U/mL. Results of the verification experiment under the optimized fermentation condi- tions showed that the enzyme activity of rhPON1 was 0.735U/mL. Human paraoxonase 1 （hPON1） is considered as an potential organophosphate pesticide scavenger which can hydrolyze various organophosphates.

关 键 词：人对氧磷酶1 巴斯德毕赤酵母 响应面方法 表达优化 

分 类 号：Q786[生物学—分子生物学]