梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用  

The Protective Effect of Zige Lyophilized Powder for Injection Against the Microvascular Contraction Function After Cerebral Ischemia-Reperfusion Injury of Rats in the Microcirculation

作　　者：尚远宏[1,2] 田金凤[1] 刘庆[1] 王敏[1] 徐晓玉[2] 

机构地区：[1]攀枝花学院干热河谷特色生物资源研发四川省高校重点实验室,四川攀枝花617000 [2]西南大学药学院/重庆市药效评价工程技术研究中心,重庆400716

出　　处：《西南大学学报（自然科学版）》2017年第6期21-27,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家青年基金项目(31402237);四川省教育厅重点基金项目(14ZA0345;15ZA0370);四川省高校重点实验室开放基金项目(GR-2013-E-02;GR-2015-E-01);攀枝花市社会发展科技计划项目(2014TX-10-3;2016CY-S-10);攀枝花学院博士科研启动经费项目(bkqj2015026;bkqj2015023)

摘　　要：探讨梓葛冻干粉针对脑缺血/再灌注损伤大鼠脑微血管舒缩功能异常的改善作用.用线栓法复制大鼠缺血/再灌注模型,分别缺血2h,于再灌注1d,4d,7d和14d给药后,取脑,按照相应试剂盒测定脑组织NOS,ET-1,TXB2和6-Ke-to-PGF1α质量分数.结果表明,与模型组相比,再灌注4d,7d和14d,梓葛冻干粉针16.40mg/kg,32.70mg/kg和65.40mg/kg能显著降低TNOS,iNOS活力,ET-1质量分数,TXB2质量分数及TXB2/6-Ke-toPGF1α的比值(p<0.01),且也能显著升高cNOS活力和6-Ke-to-PGF1α质量分数(p<0.01).得出梓葛冻干粉针有改善脑缺血/再灌注损伤大鼠微血管舒缩功能异常的作用.Objective To investigate the protective effect of Zige lyophilized powder for injection against the microvascular contraction function after cerebral ischemia-reperfusion injury of rats. Methods It made a cerebral ischemia-reperfusion model using the line in the left middle cerebral artery occlusion （MCAO） of rats in ischemia for 2 h and reperfusion for 1 d, 4 d, 7 d and 14 d, the model of rats was established by intravenous injection of drugs. Nitric oxide synthase （NOS）, endothelin-1 （ET-1）, 6-ketone-prostaglandin F1a（6-Ke-to-PG-F1a） and thromboxane B2 （TXB2） content of the brain microvessel in the lateral ischemic cerebral cortex was detected by enzyme immunoassay kits. Results In reperfusion for 4, 7 and 14 d, compared with model group, Zige lyophilized powder groups （16.40 mg/kg, 32.70 mg/kg and 65.40 mg/kg） had reduced significantly the level of TNOS, iNOS, ET-1, TXB2 and TXBz/6-Ke-to-PGF1a in microvessel of the lateral ischemic cerebral cortex （p〈0.01）, and had increased significantly the level of significantly cNOS and 6-Ke-to-PGF1a （p〈0.01）. Conclusion Zige lyophilized powder for injection can improve the effect of cerebral microcirculation. Zige lyophilized powder can protect cerebral ischemia-reperfusion injury by improving the microvascular contraction function.

关 键 词：梓葛冻干粉针 NOS ET-1 TXB2 6-Ke-to-PGF1α 脑微血管舒缩功能 

分 类 号：R969.4[医药卫生—药理学]