CD117和CD34在子宫腺肌病病灶组织中的表达研究  

Expression of CD_(117) and CD_(34) in uterine adenomyosis lesions

作　　者：郭丽群[1,2] 龙文杰 宝磊[1] 郎福地 黄凌佳 李慧[1] 金烨[1] 田海[4] 黄明莉[1] 

机构地区：[1]哈尔滨医科大学附属第一医院妇产科,150001 [2]哈尔滨医科大学附属第二医院教育部重点实验室,150086 [3]济宁市人民医院妇产科,山东272000 [4]哈尔滨医科大学附属第二医院心外科,150086

出　　处：《中华妇幼临床医学杂志（电子版）》2017年第3期326-331,共6页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金青年科学基金项目(81401203);黑龙江省自然科学基金(H201433);哈尔滨市科学技术局科技创新人才基金(2013RFLYJ003)~~

摘　　要：目的探讨CD_(117)和CD_(34)在子宫腺肌病病灶组织中的分布及CD_(117)^+细胞的数量,从而探讨CD_(117)细胞与子宫腺肌病关系。方法选择2014年7月至2016年12月在哈尔滨医科大学附属第一医院因子宫腺肌病接受子宫切除术的54例患者的子宫腺肌病病灶组织标本为研究对象,并纳入子宫腺肌病组。选择同期于本院因子宫肌瘤或者卵巢良性肿瘤行子宫切除术的50例患者的正常子宫肌层组织纳入对照组(n=54)。通过免疫组织化学染色对比研究CD_(117)在子宫腺肌病病灶组织和正常子宫肌层组织中的表达水平;通过CD_(117)、CD_(34)免疫荧光染色探讨子宫腺肌病病灶组织CD_(117)细胞与血管形成的关系。本研究遵循的程序符合哈尔滨医科大学附属第一医院人体试验委员会制定的伦理学标准,得到该委员会批准。本研究征得受试对象的知情同意,并与之签署临床研究知情同意书。结果 (1)子宫腺肌病组的病灶组织和对照组的正常子宫肌层组织中均可见CD_(117)细胞,CD_(117)细胞在子宫腺肌病组病灶组织中的表达水平高于对照组;光学显微镜下子宫腺肌病组平均每个视野的CD_(117)细胞计数为(18±4)个,对照组为(8±3)个,二者比较,差异有统计学意义(t=2.571,P<0.05)。(2)子宫腺肌病病灶组织CD_(34)、CD_(117)免疫荧光双染染色结果显示,荧光显微镜下观察CD_(117)^+细胞为CD_(117)^+/CD_(34)^-细胞。子宫腺肌病病灶组织中存在较多CD_(34)^+微小血管结构,CD_(117)细胞则大致分布于子宫腺肌病病灶的微小血管周围,血管壁的内皮细胞中可见CD_(34)^+,无CD_(117)^+表达。结论 CD_(117)^+细胞可能参与子宫腺肌病的发病机制。Objective To investigate the relationship between the distribution and quantity of CD117 in the disease tissue of adenomyosis and angiogenesis, to explore the relationship between CD117 cells and adenomyosis.Methods From July 2014 to December 2016 in the First Affiliated Hospital of Harbin Medical University, gynecological patients underwent hysterectomy for uterine myopathy in 54 patients with adenomyosis were selected as the study object and included in the uterine gland disease group.Select the same period in the hospital due to uterine fibroids or ovarian benign tumor hysterectomy in 50 patients with normal uterine muscle tissue into the control group （n=50）.The expression of CD117 in adenomyosis and normal myometrial tissue was studied by immunohistochemical staining.The relationship between CD117 cells and uterus was studied by CD117 and CD34＋ immunofluorescence staining.The procedures followed in this study are in line with the ethical standards set by the Human Body Test Committee of the First Affiliated Hospital of Harbin Medical University and are approved by the committee.The study obtained the informed consent of the subjects, and signed a clinical research informed consent.Results CD117 cells were found in the normal tissues of the adenomyosis group and the control group.CD117 cells expressed higher in the lesion tissues of the adenomyosis group than that in the control group.The average CD117 cells was （18±4）, the control group was （8±3）, the difference was statistically significant （t=2.571, P〈0.05）.② CD34＋ and CD117 immunofluorescence staining showed that CD117 positive cells were CD117/CD34-cells under fluorescence microscope.There were more CD34＋ microvascular structures in the tissues of adenomyosis, CD117 cells were distributed in the small blood vessels of adenomyosis, and CD34＋ expression was found in the endothelial cells of the vessel wall, and no positive CD117＋ expression was not found.Conclusions CD117 positive cells may be involved in the pathogenesis of

关 键 词：原癌基因蛋白质C-KIT 抗原 CD34 子宫腺肌病 血管形成 女性 

分 类 号：R711.71[医药卫生—妇产科学]