流动注射化学发光法测定利福喷丁  

Determination of Rifapentine by Flow Injection Chemiluminescence Method

作　　者：徐通[1] 张琰图[1] 张绪[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《分析测试学报》2017年第6期805-808,共4页Journal of Instrumental Analysis

基　　金：陕西省高水平大学建设专项资金(2013SXTS03);陕西省重大基础研究项目(2016ZDJC-19);延安市科技创新团队项目(2015CHTD-04)

摘　　要：基于碱性介质中利福喷丁能增敏鲁米诺-铁氰化钾体系的化学发光这一现象,结合流动注射分析技术,建立了流动注射化学发光法测定利福喷丁的新方法。在最佳实验条件下,利福喷丁检测浓度的线性范围为0.008~0.88 mg/L,检出限(3σ)为1.2×10-3mg/L。对6.0×10-2mg/L的利福喷丁进行平行测定,其相对标准偏差(RSD,n=11)为1.9%。该方法已用于测定胶囊和血浆中利福喷丁的含量,结果满意。A novel method of flow injection chemiluminescence for the determination of rifapentine was proposed based on the phenomenon that the chemiluminescence intensity of the luminol-K3Fe（CN）6 system could be enhanced by rifapentine in alkaline medium,combined with flow injection analysis technique.Under the optimal experimental conditions,the linear range of rifapentine’s detection concentration was 0.008-0.88 mg/L,the detection limit（3σ） was 1.2×10^-3 mg/L,and the relative standard deviation（RSD,n=11） was 1.9% for rifapentine at 6.0×10^-2 mg/L.The method was applied in the determination of rifapentine in both capsules and plasma with satisfactory results.

关 键 词：流动注射 化学发光 鲁米诺 铁氰化钾 利福喷丁 

分 类 号：O657.3[理学—分析化学] TQ460.72[理学—化学]