柱前衍生-高效液相色谱法测定油田采出液中的烷基胺碳酸盐型表面活性剂  

Determination of Alkylamine Carbonate Surfactant in Oilfiled Produced Liquid by HPLC with Pre-Column Derivatization

作　　者：姚峰[1] 

机构地区：[1]江苏油田石油工程技术研究院,扬州225009

出　　处：《理化检验（化学分册）》2017年第6期646-649,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：江苏省科技厅前瞻性联合研究项目(BY2015061-09);扬州市科技局市校合作项目(2014-1)

摘　　要：以自制的3,6-二甲氧基-9-苯基咔唑-1-磺酰氯(DPCS-Cl)为柱前衍生剂,采用高效液相色谱法测定油田驱油采出液中烷基胺碳酸盐型非离子-阴离子表面活性剂(C_(18)A5C)。样品经过滤去除泥沙后,分取10L浓缩至5mL,用正丁醇萃取2次后,将萃取液浓缩定容至10.0mL,加入800mg·L^(-1) DPCS-Cl乙腈溶液0.2mL,在60℃衍生反应30min,衍生物在Shim-Pack VP-ODS反相色谱柱上分离,以水-乙腈为流动相,荧光检测器的激发波长为318nm,发射波长为440nm。C_(18)A5C的线性范围为1.0~80.0mg·L^(-1),检出限为0.05mg·L^(-1)。加标回收率为91.6%~96.3%,日内和日间测定值的相对标准偏差分别为3.0%~4.9%和4.3%~5.3%。Alkylamine carbonate nonionic-anion surfaetant （C18A5C） in oilfield produced liquid was determined by HPLC with 3, 6-dimethoxy9-phenylcarbazole-l-sulfony chloride （DPCS-C1） as pre-column derivatization reagent. After being filtered to remove the sediment, 10 L of the sample was concentrated to 5 mL and then extracted with N-butanol for two times. The extract was concentrated and set to 10. 0 mL. 0. 2 mL of DPCS-C1 acetonitrile solution （800 mg · L^-1 ） was added to the extract and the mixture derivatized at 60 ℃ for ao min, the derivative was seperated on a Shim-Pack VP-ODS reverse chromatographic column, with a mixture of water and aeetonitrile as the mobile phase. The extraction wavelength and exmission wavelenght of the fluorescence detector were 318, 440 nm respectively. The linearity range of C18A5C was 1.0--80. 0 mg · L-1, with detection limit of 0. 05 mg· L^- 1. Recovery rates obtained by standard addition method in the range of 91.6%--96.3%, and the intra-day and inter-day RSDs were 3.0%-4. 9% and 4. 3%-5. 3%, respectively.

关 键 词：高效液相色谱法 衍生 表面活性剂 油田采出液 

分 类 号：O652.6[理学—分析化学]