α-倒捻子素脂质体的药动学研究  

Study on Pharmacokinetics of α-Mangostin Lipsome

作　　者：吴飞跃 林玳 顾卓珺 郑怀宇 傅军[2] 

机构地区：[1]舟山医院药剂科,浙江舟山316000 [2]同济大学附属第十人民医院药学部,上海200072

出　　处：《中国现代应用药学》2017年第5期703-705,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：舟山市医药卫生科技计划(2015G02)

摘　　要：目的采用LC-MS/MS考察α-倒捻子素脂质体的药动学特征。方法采用SPF级ICR小鼠,♀,尾静脉注射α-倒捻子素脂质体3 mg·kg-1,用LC-MS/MS测定α-倒捻子素血药浓度,并计算其药动学参数。结果α-倒捻子素线性范围为5~1 000 ng·m L^(-1)(r=0.997 6),定量限为5 ng·m L^(-1),日内、日间精密度均<11.0%。主要药动学参数:t1/2为(0.99±0.12)h,Cmax为(1 524.60±323.82)ng·m L^(-1),AUC0-t为(645.84±63.74)ng·h·m L^(-1),MRT为(0.70±0.11)h,CL为(4.80±0.45)m L·h^(-1)·kg^(-1),Vd为(6.80±1.00)m L·kg^(-1)。结论该法操作简便、快速、灵敏,可用于测定小鼠血浆中α-倒捻子素浓度。本研究结果可为药物的进一步研究提供依据。OBJECTIVE To establish a sensitive and specific LC-MS/MS method for investigating the pharmacokinetics characteristic of α-mangostin liposome in mice. METHODS ICR mice were given in tail vein α-mangostin 3 mg·kg-1. The α-mangostin concentration was determined by LC-MS/MS and pharmacokinetic parameters was calculated. RESULTS The linearity ranged from 5 to 1 000 ng·mL^-1 （r=0.997 6）. The detectction of α-mangostin was 5 ng·mL^-1. The RSDs within day and between days were 〈11%. The main pharmacokinetic parameters of t1/2, Cmax, AUC0-t, MRT, CL and Vss were （0.99±0.12）h, （1 524.60±323.82）ng·mL-1, （645.84±63.74）ng·h·mL-1, （0.70±0.11）h, （4.80±0.45）mL·h-1·kg-1, and （6.80±1.00）mL·kg^-1, respectively. CONCLUSION The precision, accuracy and recovery of the method are found to be adequate for pharmacokinetic studies. The results can provide the basis for future research.

关 键 词：α-倒捻子素 脂质体 液相色谱-质谱 药动学 

分 类 号：R969.1[医药卫生—药理学]