拟南芥磷酸肌醇特异性磷脂酶C(AtPLC2)的亚细胞定位分析  

Subcellular localization of phosphoinositide-specific phospholipase C(AtPLC2) gene in Arabidopsis thaliana

作　　者：赵亚婷[1] 韩玉霞 邢红侠 李玉琦[1] 庞茜[1] 邢继红[1] 董金皋[1] 

机构地区：[1]河北农业大学,真菌毒素与植物分子病理学实验室,河北保定071000 [2]张家口东方中学,河北张家口075000

出　　处：《河北农业大学学报》2017年第3期38-41,58,共5页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2014405010)

摘　　要：为明确拟南芥磷酸肌醇特异性磷脂酶C(AtPLC2)的亚细胞定位情况,本研究构建了AtPLC2基因融合GFP标签的表达载体35S:PLC2-GFP,并通过农杆菌介导的遗传转化方法,将重组质粒转化本氏烟草叶片,转基因烟草叶片细胞利用激光扫描共聚焦显微镜观察,绿色荧光蛋白荧光信号与细胞质膜重合,表明拟南芥AtPLC2基因定位于细胞质膜上,研究结果为明确拟南芥AtPLC2基因的功能奠定了良好的基础。To clarify the subcellular localization of AtPLC2 gene in Arabidopsis thaliana, the AtPLC2 gene was cloned and fused with GFP in frame to construct a binary vector 35S.PLC2- GFP. Then,the binary vector was transformed into Nicotiana bentharniana by Agrobacterium tumefaciens-mediated method. Using confocal laser scanning microscopy, green fluorescent signals were observed on plasma membrane of the transformed Nicotiana benthamiana cells. These results indicated that the AtPLC2 gene of Arabidopsis thaliana was localized on plasma membrane.

关 键 词：拟南芥 AtPLC2 绿色荧光蛋白 亚细胞定位 

分 类 号：S432.23[农业科学—植物病理学]