七氟醚预处理对单肺通气患者术侧肺组织自噬的影响  

Effect of sevoflurane preconditioning on autophagy in operated lung tissues of patients undergoing one-lung ventilation

作　　者：韩杨杨[1] 朱浩[2] 张凯[1] 王妍[1] 黄长顺[2] 方向明[1] 

机构地区：[1]浙江大学医学院附属第一医院麻醉科,杭州市310003 [2]宁波市第一医院麻醉科,315010

出　　处：《中华麻醉学杂志》2017年第4期417-419,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价七氟醚预处理对单肺通气患者术侧肺组织自噬的影响。方法择期全麻下行胸腔镜下肺癌根治术患者40例，ASA分级Ⅰ-Ⅲ级，性别不限，年龄30～80岁，体重45～84 kg，采用随机数字表法分为2组（n＝20）：对照组（C组）和七氟醚预处理组（SP组）。SP组于单肺通气前吸入七氟醚，呼气末浓度为2.0%，吸入30 min后洗出。取距离肿块边缘〉2 cm的正常肺组织，采用Western blot法检测微管相关蛋白1轻链3Ⅱ的表达水平。结果与C组比较，SP组肺组织微管相关蛋白1轻链3Ⅱ表达上调（ P〈0.05）。结论七氟醚预处理可增强单肺通气患者术侧肺组织自噬，该作用可能与其肺保护效应有关。Objective To evaluate the effect of sevoflurane preconditioning on autophagy in the operated lung tissues of the patients undergoing one-lung ventilation.Methods Forty patients of both sexes, with American Society of Anesthesiologists physical status Ⅰ-Ⅲ, aged 30-80 yr, weighing 45-84 kg, scheduled for elective radical operation for lung cancer performed via video-assisted thoracoscope under general anesthesia, were divided into control group（C group）and sevoflurane preconditioning group（SP group）, with 20 patients in each group.In SP group, sevoflurane was inhaled for 30 min with the final concentration of 2.0%, followed by washout.The normal lung tissues（the distance from the edge of the lump〉2 cm）around the lump were obtained for detection of the expression of microtubule-associated protein 1 light chain 3 Ⅱ by Western blot.Results Compared with C group, the expression of microtubule-associated protein 1 light chain 3 Ⅱ in lung tissues was significantly up-regulated in SP group（P〈0.05）.Conclusion Sevoflurane preconditioning can enhance autophagy in the operated lung tissues of the patients undergoing one-lung ventilation, which may be related to the lung protective effect.

关 键 词：麻醉药 吸入 缺血预处理 自噬 呼吸 人工 呼吸窘迫综合征 成人 

分 类 号：R614[医药卫生—麻醉学]