皂角刺黄酮和香豆素类活性成分的体外抗癌活性筛选  

Screening of Anticancer Activity of Gleditsia Sinensis Flavonoids and Coumarins of Active Ingredients

作　　者：杨櫹[1] 谭文霞[2] 王婷婷[2] 高嘉屿[2] 

机构地区：[1]河南科技大学临床医学院,河南洛阳471023 [2]河南科技大学化工与制药学院,河南洛阳471023

出　　处：《中国继续医学教育》2017年第12期215-217,共3页China Continuing Medical Education

基　　金：河南科技大学SRTP项目(2016056);2016国家级大学生创新创业训练计划项目(201610464031)

摘　　要：目的系统筛选由皂角刺获得的黄酮以及香豆素类化合物的体外抗癌活性。方法使用MTT细胞毒性法,首先在单一浓度(100μg/ml)下筛选18种化合物在两种癌细胞株上的活性,继而选择活性强的化合物进行浓度梯度的细胞毒实验,并计算IC50。结果异牡荆素,5-甲基氧-3,’4’,7-三羟基二氢黄酮醇,滨蒿内酯和异东莨菪内脂活性较强,可对癌细胞产生浓度相关的生长抑制作用。结论黄酮和香豆素类化合物构成了皂角刺抗癌的成分基础。Objective The screening system in vitro antitumor activity of thorn obtained flavonoids and coumarins from saponins. Methods The use of MTT cytotoxicity method, first in a single concentration （100 g/ml） under the screening of 18 compounds in two kinds of cancer cell lines activity, cytotoxicity test and selection of compounds with strong activity concentration gradient, and calculate the IC50. Results Isovitexin, 5- methyl oxygen -3, ＇4＇, 7- three hydroxy two flavanonols, lipid activity of Scoparone and scopoletin in strong, can inhibit growth of cancer cell concentration. Conclusion Flavonoids and coumarins constitute the basis of anticancer components of Gleditsia sinensis.

关 键 词：皂角刺黄酮 香豆素 抗癌 

分 类 号：R3[医药卫生—基础医学]