Structure and activity of thermophilic methanogenic microbial communities exposed to quaternary ammonium sanitizer  

作　　者：Jesus D.Fernandez-Bayo Juliano Toniato Blake A.Simmons Christopher W.Simmons 

机构地区：[1]Department of Food Science and Technology,University of California,Dauis,CA 95616,USA [2]Department of Biological and Agricultural Engineering,Uniuersity of California,Dauis,CA 95616,USA [3]Joint BioEnergy Institute,Emeryville,CA 94608,USA [4]Biological and Engineering Sciences Center,Sandia National Laboratories,Liuermore,CA 94551,USA [5]Energy Efficiency Center,University of California,Dauis,CA 95616,USA

出　　处：《Journal of Environmental Sciences》2017年第6期164-168,共5页环境科学学报（英文版）

基　　金：supported by the Office of Science of the U.S.Department of Energy under Contract No.DE-AC02-05CH11231

摘　　要：Food processing facilities often use antimicrobial quaternary ammonium compound （QAC） sanitizers to maintain cleanliness. These QACs can end up in wastewaters used as feedstock for anaerobic digestion. The aim of this study was to measure the effect of QAC contamination on biogas production and structure of microbial communities in thermo- philic digester sludge. Methane production and biogas quality data were analyzed in batch anaerobic digesters containing QAC at 0, 15, 50, 100 and 150 mg/L. Increasing sanitizer concentration in the bioreactors negatively impacted methane production rate and biogas quality. Microbial community composition data was obtained through 16S rRNA gene sequencing from the QAG-contaminated sludges. Sequencing data showed no significant restructuring of the bacterial communities. However, significant restructuring was observed within the archaeal communities as QAC concentration increased. Further studies to confirm these effects on a larger scale and with a longer retention time are necessary.Food processing facilities often use antimicrobial quaternary ammonium compound （QAC） sanitizers to maintain cleanliness. These QACs can end up in wastewaters used as feedstock for anaerobic digestion. The aim of this study was to measure the effect of QAC contamination on biogas production and structure of microbial communities in thermo- philic digester sludge. Methane production and biogas quality data were analyzed in batch anaerobic digesters containing QAC at 0, 15, 50, 100 and 150 mg/L. Increasing sanitizer concentration in the bioreactors negatively impacted methane production rate and biogas quality. Microbial community composition data was obtained through 16S rRNA gene sequencing from the QAG-contaminated sludges. Sequencing data showed no significant restructuring of the bacterial communities. However, significant restructuring was observed within the archaeal communities as QAC concentration increased. Further studies to confirm these effects on a larger scale and with a longer retention time are necessary.

关 键 词：Anaerobic digestionAntimicrobialsBiofuelsMicrobial ecologyWaste management 

分 类 号：S216.4[农业科学—农业机械化工程] X703[农业科学—农业工程]