不明原因早期复发性流产患者蜕膜自然杀伤细胞对滋养层细胞系侵袭功能的影响  

Impact of decidual natural killer(NK)cells of unexplained recurrent spontaneous abortion in pro-invasion capability of HTR-8/SVneo

作　　者：郭雯娓 李博[1] 肖西峰[1] 陈书强[1] 王珺[1] 陈丽华[2] 王晓红[1] 

机构地区：[1]第四军医大学唐都医院生殖医学中心,西安710038 [2]第四军医大学免疫学教研室,西安710032

出　　处：《中华生殖与避孕杂志》2017年第5期378-383,共6页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金面上项目(81370710;81571531)~~

摘　　要：目的研究早孕期复发性流产(RSA)患者与正常早孕妇女的蜕膜自然杀伤细胞(d NK)对滋养层细胞系HTR-8/SVneo侵袭能力的影响。方法收集正常早孕人工流产妇女(正常早孕组,n=10)和不明原因RSA患者(RSA组,n=8)的早孕蜕膜组织,用密度梯度离心法分离出蜕膜淋巴细胞,免疫磁珠法进一步分选出CD56+CD16-NK细胞,即蜕膜NK细胞,将蜕膜NK细胞与HTR-8/SVneo共培养24 h后,通过MTT法和Transwell侵袭实验检测蜕膜NK细胞对滋养层细胞系HTR8/SVneo黏附及侵袭能力的影响,用Real-time PCR检测共培养后HTR-8/SVneo细胞的MMP-2和MMP-9 mRNA水平的表达情况。结果与正常早孕妇女相比,不明原因早孕期RSA患者的蜕膜NK细胞对滋养层细胞系HTR8/SVneo侵袭能力有减弱作用,并使HTR-8/SVneo表达促侵袭分子MMP-2和MMP-9表达降低。结论妊娠早期局部母-胎界面d NK细胞的功能异常可能是导致不明原因RSA的一个原因。Objective To investigate the impact of decidual natural killer （dNK） cells in pro-invasion capability of HTR-8/SVneo between recurrent spontaneous abortion （RSA） and normal pregnancy female.Methods Decidual lymphocytes from early pregnancy decidua tissue of RSA and normal pregnancy female were obtained by the density gradient centrifugation, furthermore, immunomagnetic beads were used to separate CD56＋CD16-NK cells. In addition, the adhesion and pro-invasion capability of HTR-8/SVneo were evaluated by MTT and Transwell respectively after co-culture dNK cells and HTR-8/SVneo for 24 h. Real-time PCR was used to assess the expression of MMP-2 and MMP-9 mRNA in HTR-8/SVneo.Resultsd NK cells from RSA weaken the pro-invasion capability of HTR-8/SVneo, accompanying by down-regulating the expression of MMP-2 and MMP-9.Conclusion Dysfunction of dNK cells in fetal-maternal interface may lead to RSA.

关 键 词：蜕膜自然杀伤细胞(dNK) 复发性流产(RSA) 滋养层细胞 侵袭 

分 类 号：R714.21[医药卫生—妇产科学]