淋巴细胞主动免疫治疗原因不明性不孕IVF患者的疗效观察  

Effectiveness of lymphocyte immunotherapy for patients with unexplained infertility before in vitro fertilization-embryo transfer

作　　者：王飞艳[1] 王勇[1] 王蕾[1] 曾琼芳[1] 蒋元华[1] 段金良[1] 

机构地区：[1]中国人民解放军第一八一医院生殖中心,广西桂林541002

出　　处：《中国性科学》2017年第6期146-149,共4页Chinese Journal of Human Sexuality

基　　金：广西科学研究与技术开发计划项目(桂科攻1140003A-38)

摘　　要：目的:探讨淋巴细胞主动免疫治疗对不明原因不孕患者体外受精-胚胎移植(IVF-ET)妊娠结局的影响。方法:随机选取2011年5月至2015年9月在本院生殖中心进行IVF助孕、封闭抗体(BA)阴性的462例不明原因性不孕患者作为研究对象,按照患者是否行免疫治疗分为治疗组(187例)和对照组(275例),应用流式细胞仪检测两组患者外周血BA(抗CD3^+、CD4^+、CD8^+)的封闭效率情况,观察淋巴细胞主动免疫治疗IVF-ET患者的妊娠结局。结果:免疫治疗组患者血清抗CD3^+、CD4^+、CD8^+的表达率明显高于对照组(P<0.05);免疫治疗组患者的胚胎着床率、临床妊娠率均明显高于对照组(P<0.05);而两者的流产率、获卵数、受精率、优胚数及移植胚胎数比较,其差异均无统计学意义(P>0.05)。结论:淋巴细胞主动免疫治疗有助于改善BA阴性的不明原因不孕患者IVF-ET的妊娠结局,是治疗原因不明性不孕的一种有效方法。Objectives： To investigate the effect of lymphocyte immunotherapy on patients with unexplained infertility undergoing in vitro fertilization-embryo transfer（IVF-ET）. Methods： 462 patients diagnosed as unexplained infertility undergoing IVF caused by lacking blocking antibodies（BA） from May 2011 to September 2015 were selected and randomly divided into two groups,treatment group（n = 187） and control group（n = 275）. The percentages of anti-CD-3 ＋,CD4 ＋ and CD8 ＋ were examined by flow cytometry,and lymphocytes immunotherapy was performed in the treatment group. Pregnancy outcome was compared between the two groups. Results：The expression rate of anti-CD3 ＋,CD4 ＋ and CD8 ＋ in the peripheral blood in active immunotherapy were statistically higher than that in the control group（P〈0. 05）. The pregnancy rate and implantation rate in the treatment group was higher（P〈0. 05）. There was no significant difference in the miscarriage rate,the number of retrieved oocytes,fertilization rate,cleavagerate,embryo grading and the number of embryos transplanted between the twogroups（P〉0. 05）. Conclusion： Lymphocyte immunotherapy can improve the outcome of IVF-ET in the patient with unexplained infertility,an effective method of treating unexplained infertility.

关 键 词：淋巴细胞主动免疫治疗 不明原因不孕患者 IVF-ET 封闭抗体 

分 类 号：R71[医药卫生—妇产科学]