基于抗心肌缺血作用的金花葵制备物药物质量表征分析  

Drug Quality Representation Analysis of Preparations of Aurea Helianthus based on Anti-myocardial Ischemia Effect

作　　者：谭立[1] 孔静[1] 冯朵[1] 刘璐[1] 吕珊[1] 张元元[1] 石任兵[1,2] 

机构地区：[1]北京中医药大学中药学院/国家中医药管理局中药经典名方有效物质发现重点实验室,北京100102 [2]北京市教委中药质量控制技术工程中心,北京100102

出　　处：《世界科学技术-中医药现代化》2017年第3期522-528,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：北京中医药大学重点学科开放课题(2013-ZDXKKF-23):中药特性化学;负责人:石任兵

摘　　要：目的:优选金花葵最佳药物制备物形式,为金花葵抗心肌缺血药物的研究开发提供科学依据。方法:建立HPLC-PDA法测定金花葵制备物有效指标性成分的含量。抗心肌缺血作用考察采用垂体后叶素致大鼠急性心肌缺血模型。对金花葵制备物进行基于药学、药效的药物质量表征及关联分析。结果:所建立HPLC含量测定方法通过方法学验证,平均加样回收率在97.04%-102.44%之间;金花葵3种制备物均表现出不同程度的抗心肌缺血作用,其中富集物抗心肌缺血作用最强,而得率最低、药物用量最小,为金花葵抗心肌缺血药物最佳制备物形式。结论:基于药物体系架构的金花葵制备物富集物,有关药物成分的含量较高、相对比值较为合理,呈现优质药物质量表征。因此,基于药物体系导向药物制备,有针对性地提高有关药物成分含量、探寻其最佳组成比例是提升中药药物药效的关键。This study was aimed to select the best preparation form of Aurea Helianthus in order to provide a scientific basis for research and development of anti-myocardial ischemia drugs. The content of effective index components of preparation of Aurea Helianthus was detected using HPLC-PDA. Acute myocardial ischemia rat model introduced by injecting pituitrin was used to investigate the effect of anti-myocardial ischemia. The quality of preparation from Aurea Helianthus was characterized based on pharmacy and efficacy. And the characterized results were given correlation analysis. The results showed that the HPLC method for quantitative determination was verified by methodology. The average recoveries were in the range of 97.04%- 102.44%. All three preparations of Aurea Helianthus had the efficacy of anti-myocardial ischemia in varying degrees. The enrichment had the highest effect with lowest extraction rate and the lowest dosage, which was the best form in medicinal-preparation application of Aurea Helianthus. It was concluded that the content of related drug components was higher with unique proportion in enrichment of Aurea Helianthus which was prepared based on drug system proved a high quality medicine. Therefore, the key to the improvement of efficacy was to explore the fittest proportion and improve the content of related pharmaceutical ingredients pertinently according to drug system.

关 键 词：金花葵制备物 抗心肌缺血 质量表征 药学-药效关联分析 

分 类 号：R282.5[医药卫生—中药学]