KiSS-1和VEGF在涎腺黏液表皮样癌中的表达及其对血管生成的影响  

Expression of KiSS-1 and VEGF in mucoepidermoid carcinoma of salivary glands and its effect on angiogenesis

作　　者：马赛[1] 安峰[1] 胥爱文[1] 王芹[1] 刘博[2] 

机构地区：[1]河北北方学院附属第一医院口腔科,河北张家口075000 [2]河北北方学院附属第一医院病理科,河北张家口075000

出　　处：《海南医学》2017年第10期1555-1559,共5页Hainan Medical Journal

基　　金：河北省医学科学研究重点课题项目计划项目(编号:20150059);河北省张家口市科学技术研究与发展指导计划项目(编号:1421128D);河北省政府(2015)资助临床医学优秀人才培养和基础课题研究项目(编号:361009)

摘　　要：目的研究肿瘤转移抑制基因KiSS-1和血管内皮生长因子(VEGF)在涎腺黏液表皮样癌(MEC)组织中的表达情况及其对血管生成的影响。方法选择河北北方学院附属第一医院口腔科2007年6月至2016年6月收治的68例MEC患者为研究对象。应用免疫组织化学SP法检测68例MEC组织、20例正常涎腺组织中KiSS-1和VEGF的表达,统计学比较其在不同临床病理特征的MEC组织中表达阳性率的差异。光镜下计数利用CD105标记的微血管密度(MVD),统计学比较不同临床病理特征的MEC组织MVD值的差异,并观察KiSS-1和VEGF的表达对MEC组织MVD值的影响。结果 68例MEC组织中KiSS-1的阳性表达率为38.24%,明显低于正常涎腺组织的70.00%,VEGF的阳性表达率为70.59%,明显高于正常涎腺组织的10.00%,差异均有统计学意义(P<0.05)。KiSS-1的表达与MEC的淋巴结转移和TNM分期相关(P<0.05),VEGF的表达与MEC的病理分级、淋巴结转移和TNM分期相关(P<0.05);KiSS-1阳性组MVD值为(29.50±9.14),明显低于阴性组的(35.24±10.45),VEGF阳性组MVD值为(35.33±10.20),明显高于阴性组的(27.57±8.45),差异均有显著统计学意义(P<0.01);KiSS-1的表达与VEGF表达呈负相关(r_s=-0.488,P=0.000)。结论在MEC组织中KiSS-1表达的下调可能促进VEGF的表达,从而促进了MEC组织血管的生成,促进其侵袭和转移。Objective To investigate the relationship of the expression of tumor metastasis suppressor gene kisspeptin-1 （KiSS-1） and vascular endothelial growth factors （VEGF） with angiogenesis in mucoepidermoid carcinoma （MEC） of salivary glands. Methods A total of 68 MEC patients, who admitted to Department of Stomatology of the First Affiliated Hospital of Hebei North College from June 2007 to June 2016, were chosen as research objects. The ex-pression of KiSS-1 and VEGF was detected with immunohistochemical method （SP） in 68 cases of MEC tissues and 20 cases of normal salivary gland. Statistical method was used to compare the differences of expression positive rates of KiSS-1 and VEGF in different clinical pathological types of MEC. The microvessel density （MVD） was recorded under optical microscope. Statistical method was used to compare the differences of MVD in different clinical pathological types of MEC, and to observe how the expression of KiSS-1 and VEGF affect the MVD in MEC. Results The posi-tive expressions of KiSS-1 in MEC tissues was 38.24%, which was significantly lower than 70.00%in the normal sali-vary gland group （P〈0.05）. The positive expressions of VEGF in MEC tissues was 70.59%, which was significantly higher than 10.00%in the normal salivary gland group （P〈0.05）. The expression of KiSS-1 was correlated with lymph node metastasis and TNM staging （P〈0.05）;the expression of VEGF was correlated with pathological grade level, lymph node metastasis, TNM staging （P〈0.05）. The MVD in KiSS-1 positive expression group was （29.50 ± 9.14）, which was significantly lower than （35.24 ± 10.45） in KiSS-1 negative expression group （P〈0.05）;the MVD in VEGF positive ex-pression group was （35.33±10.20）, which was significantly higher than （27.57±8.45） in VEGF negative expression group （P〈0.01）. The expression of KiSS-1 was negatively correlated with VEGF （rs=-0.488, P=0.000）. Conclusion The down-regulation expression of KiSS-1 in MEC of saliv

关 键 词：涎腺 黏液表皮样癌 KISS-1 血管内皮生长因子 细胞膜糖蛋白 微血管生成 

分 类 号：R736[医药卫生—肿瘤]