生理性孕酮撤退过程中小鼠子宫角组织蛋白激酶B、糖原合成激酶3β的表达观察  

Expression of protein kinase B and glycogen synthase kinase 3β in mouse uterine horn during the process of physiological progesterone withdrawal

作　　者：时灿灿[1] 于明明[1] 张先平[2] 陈丽刚[1] 王乾兴[1] 

机构地区：[1]遵义医学院细胞生物学教研室,贵州遵义563003 [2]娄底市中心医院

出　　处：《山东医药》2017年第19期9-12,共4页Shandong Medical Journal

基　　金：贵州省科技厅联合基金项目(黔科合J字LKZ-2010-33号);贵州省科学技术基金资助项目(黔科合J字-2011-2282)

摘　　要：目的观察生理性孕酮撤退小鼠子宫角组织中蛋白激酶B(Akt)及糖原合成激酶3β(GSK3β)的表达变化。方法 90只未交配健康成年雌性C57BL/6J小鼠,制作生理性孕酮撤退模型,分别在孕酮撤退0、8、12、16、20、24、32、40、48 h(48 h为小鼠生理性孕酮撤退周期)时处死10只小鼠,收集子宫角组织。采用real-time PCR法检测孕酮撤退各时点小鼠子宫角组织Akt、GSK3β mRNA,采用Western blotting法检测孕酮撤退各时点小鼠子宫角组织总蛋白激酶B(t-Akt)、磷酸化蛋白激酶B(Akt)及3βp-Akt及磷酸化糖原合成激酶(p-GSK3β)。结果随孕酮撤退时间延长,Akt mRNA及蛋白相对表达量逐渐降低,孕酮撤退20 h时达到最低值,后随孕酮撤退时间逐渐延长,Akt mRNA及蛋白相对表达量逐渐升高。孕酮撤退8 h时GSK mRNA及蛋白相对表达量高于孕酮撤退0 h时(P<0.05);随孕酮撤退时间延长,GSK mRNA及蛋白相对表达量逐渐降低,孕酮撤退24 h时达到最低值,后随孕酮撤退时间逐渐延长,GSK mRNA及蛋白相对表达量逐渐增加。孕酮撤退20 h小鼠子宫角组织Akt、GSK3β mRNA表达量低于孕酮撤退后0、48 h(P均<0.05)。与孕酮撤退0 h比较,孕酮撤退20 h时小鼠子宫角组织p-Akt、p-GSK3β的相对表达量均降低(P均<0.05)。与孕酮撤退8 h比较,孕酮撤退20 h时小鼠子宫角组织p-GSK3β的相对表达量降低(P<0.05)。与孕酮撤退20 h比较,孕酮撤退48 h时小鼠子宫角组织p-Akt、p-GSK3β的相对表达量均升高(P均<0.05)。结论生理性孕酮撤退小鼠子宫角组织中存在Akt表达随时间下降后上调,GSK3β表达随时间上调后下降后再次上调。Akt/GSK-3β信号通路可能在鼠孕酮撤退过程中起重要作用。Objective To observe the expression of protein kinase B （Akt） and glycogen synthase kinase 3β （GSK3β） in the uterine tissues of mouse models inthe process of physiological progesterone withdrawal. Methods We selected 90 non-mating healthy adult female C57BL/6J mice to make the physiological progesterone withdrawal models. Ten mice were killed at 0, 8, 12, 16, 20, 24, 32, 40, and 48 h （ The physiological progesterone withdrawal cycle in mice is 48 h） to collect uterine horns. The gene expression levels of Akt and GSK3β were detected by real-time PCR. The protein expression levels of total Akt, phosphorylated AKT and phosphorylated GSK-3β were detected by Western blotting. Results Over the time of progesterone withdrawal, the expression of Akt mRNA and protein gradually decreased （ reached the minimum at 20 h）and then gradually increased. The expression of GSK3β mRNA and protein at 8 h was higher than that at 0 h （ P 〈 0.05 ）. Over the time of progesterone withdrawal, the expression of GSK mRNA and protein decreased （ reached the minimum at 24 h） and then gradually increased. The expression of Akt and GSK3β mRNA at 20 h was lower than that at 0 and 48 h （both P 〈0.05）. The expression of p-Akt and p-GSK-3β at 20 h decreased as compared with that at 0 h （both P 〈 0.05 ）. The expression of p-GSK-3β protein m mouse uterine tissues at 20 h decreased as compared with that at 8 h （ P 〈 0.05 ）. The expression of p-Akt and p-GSK3β protein at 48 h increased as compared with that at 20 h （ all P 〈 0.05）. Conclusions Over the time of progesterone withdrawal, the expression of Akt gradually decreases and then in-creases in the uterine tissues of mouse models. Meanwhile, the expression of GSK3β first increases, then decreases, and finally increases again. Akt/GSK3β signal pathway may play an important role in the process of progesterone withdrawal.

关 键 词：月经 孕酮撤退 蛋白激酶B 糖原合成激酶3β 动物实验 

分 类 号：R711.3[医药卫生—妇产科学]