神经生长因子增强间充质干细胞诱导CD4＋CD25＋Treg细胞分化和增殖的作用  

Role of nerve growth factor in the differentiation and proliferation of regulatory T cells induced by mesenchymal stem cells

作　　者：龙旺[1] 石炳毅 肖漓 谢续标[1] 彭风华[1] 蓝恭斌[1] 余少杰[1] 王彧[1] 代贺龙 高陈[1] 彭龙开[1] 

机构地区：[1]中南大学湘雅二医院泌外器官移植科,长沙410000 [2]解放军第三○九医院全军器官移植研究所,北京市器官移植与免疫调节重点实验室,北京210009

出　　处：《中华器官移植杂志》2017年第2期112-115,共4页Chinese Journal of Organ Transplantation

摘　　要：目的 研究神经生长因子（NGF）对间充质干细胞（MSC）诱导CD4＋ CD25＋调节性T淋巴细胞（Treg细胞）分化增殖能力的影响.方法 于MSC中加入NGF直接进行刺激,检测白细胞介素10（IL-10）、转化生长因子-β（TGF-β）和HLA-G等含量分析NGF对MSC的作用;直接共培养后采用流式细胞检测CD4和CD25表达;应用Transwell小室共培养后采用流式细胞检测淋巴细胞CD4、CD25、Foxp3的表达.所有检测以经NGF刺激的hBMSC作为NGF组,以未经NGF处理的hBMSC作为对照组.结果 与对照组相比,NGF组hBMSC培养上清液中IL-10和TGF-β表达均显著增加,两组比较,差异有统计学意义（P＜0.05）.流式细胞检测结果显示,与对照组相比,NGF组hBMSC的HLA-G表达显著增加（P＜0.05）;共培养后,NGF组较对照组CD4＋ CD25＋ Treg细胞的数量显著增多（P＜0.05）.抑制IL-10和TGF-p能够阻断NGF的诱导作用（P＜0.05）.结论 NGF能够增强MSC诱导Treg细胞分化增殖的能力,这种能力与MSC表达IL-10和TGF-p的增多有关.Objective To investigate the influence of nerve growth factor （NGF） on the ability of differentiation and proliferation of regulatory T cells （Tregs） induced by mesenchymal stem cells （MSCs）.Methods The MSCs were stimulated directly by NGF.IL-10,TGF-β and HLA-G were tested.The expression of CD4 and CD25 was detected by flow cytometry after co-culture.The expression of CD4,CD25 and Foxp3 was detected by flow cytometry after Transwell co-culture.Results As compared with control group,the expression of IL-10,TGF-β and HLA-G in NGF group was increased （P〈0.05 for all）.The number of Tregs was increased after the co-culture （P〈0.05）.The reduction in IL-10 and TGF-β could block the inducing function of NGF （P〈0.05）.Conclusion NGF can enhance the ability of differentiation and proliferation of Tregs induced by MSC,which is possibly associated with the increases in the expression of IL-10 and TGF-β.

关 键 词：神经生长因子 间质干细胞 调节性T淋巴细胞 免疫耐受 

分 类 号：R392[医药卫生—免疫学]