羽叶三七的转录组测序与三萜皂苷生物合成的关键酶基因的识别  

Transcriptome Sequencing of Panax pseudoginseng and Identification of Key Enzyme Genes in Triterpenoids Saponin Biosynthesis

作　　者：黄勋[1] 金健[1] 余洁[1] 韩喆[1] 闫祺[1] 陈平[1] 

机构地区：[1]武汉轻工大学生物与制药工程学院,武汉430023

出　　处：《基因组学与应用生物学》2017年第6期2531-2538,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(No.81274023)资助

摘　　要：羽叶三七是五加科人参属的名贵药材,三萜皂苷为羽叶三七最主要的活性成分。为了探索羽叶三七根茎中皂苷物质生物合成的分子基础,采用Illumina Hi Seq 2000高通量测序获得羽叶三七根茎的转录组数据;使用Trinity和TGICL软件实现Uni Gene的de novo拼接;基于BLAST完成Uni Gene的蛋白功能注释、KOG功能注释、GO分类和KEGG代谢通路分析。最终通过de novo拼接注释得到Uni Gene 62 240个。研究发现,羽叶三七根茎部表达的26个Uni Gene与三萜碳环骨架合成相关;三萜合成通路中的关键酶FPS、SS、SE等,分别有11 114个Uni Gene。该研究发现的三萜皂苷合成相关候选基因对于阐明羽叶三七三萜皂苷合成方式研究提供了理论基础。Panax pseudoginseng is a valuable Chinese herbal medicine, belonging to Araliaceae family, Panax genus. Triterpene saponins are the most active components in Panax pseudoginseng. In order to explore the molecule basis of triterpenoid saponins biosynthesis in Panax pseudogirtseng, the transcriptome data of rhizome of Panax pseudoginseng was obtained by Illumina HiSeq 2000 high-throughput sequencing, and de novo splicing of UniGene was realized by Trinity and TGICL. The protein function annotation, KOG functional annotation, GO classification and metabolism of KEGG metabolic pathway analysis of UniGene were completed based on BLAST. 62 240 UniGenes were finally obtained by de novo splicing and annotation. After annotation, we discovered 26 UniGenes expressed in rhizome of Panax pseudoginseng were involved in the biosynthesis of ginsenoside backbone. Additionally, there were 11 114 UniGenes of the key enzymes FPS, SS, SE in triterpene saponins synthesis pathway. In conclusion, the related candidate genes of triterpenoid saponin synthesis found in this study would provide a theoretical basis for elucidating the synthesis oftriterpenoid saponin in Panax pseudogimeng.

关 键 词：羽叶三七 转录组测序 三萜合成关键酶 

分 类 号：Q943.2[生物学—植物学] S567.5[农业科学—中草药栽培]