中国小反刍兽疫病毒M基因序列分析  

Sequence Analysis of M Gene of Peste Des Petits Ruminants Virus in China

作　　者：张路瑶[1] 马世强[1] 李飞[1] 张保军[1] 赵丽[1] 刘永宏[1] 

机构地区：[1]塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《塔里木大学学报》2017年第2期21-27,共7页Journal of Tarim University

基　　金：国家大学生创新创业训练计划项目(201510757011);塔里木大学动物科学学院大学生创新项目(DKY2014004;DKY2014009);华中农业大学塔里木大学科研联合基金项目(HNTDLH1404)

摘　　要：为了分析中国PPR的流行特征,从Genbank数据库中下载PPRV M基因核苷酸全序列及其对应的氨基酸序列,应用DNAStar分子生物学软件对其进行遗传演化分析。结果显示,中国2013~2014年PPRV毒株间M基因核苷酸同源性范围为99.8%~100%,与西藏3个PPRV毒株M基因核苷酸同源性范围为97.9%~98.1%,与国外PPRV毒株M基因核苷酸同源性范围为89.5%~98.2%,即核苷酸同源性最高的为印度Sungri 1996 MSD株。中国2013~2014年PPRV毒株间M蛋白氨基酸同源性为100%,与西藏3个PPRV毒株M蛋白氨基酸同源性均为97%,与国外PPRV毒株M蛋白氨基酸同源性范围为96.7%~100%。M基因核苷酸进化树分析显示,中国2013~2014年PPRV毒株与西藏的3个毒株不在同一分支,与印度毒株关系较近。推断中国2013~2014年PPR疫情非西藏2007年疫情的延续,可能为印度等周边国家传入。In order to analyze the epidemiological characteristics of China PPR, the whole sequence of nucleotide and corresponding a- mino acid sequence of PPRV M gene were downloaded from Genbank database, the nucleotide sequence of M PPRV gene and its corre- sponding amino acid sequences and the genetic evolution of gene was analyzed by using DNAStar molecular biological software. The re- suits showed that PPRV strain 2013 - 2014 China nucleotide homology of M gene between the range of 99.8% ~ I00%, and Tibet 3 PPRV strains M gene nucleotide homology ranged from 97. 9% ~ 98. 1% , compareed with foreign PPRV strains M gene nucleotide ho- mology in the range of 89. 5% ~98. 2% , which is the most high homology to the India Sungri 1996 MSD strains. The amino acid homology of M protein between PPRV and 2013 - 2014 in China was 100%, and the amino acid homology of M protein of 3 PPRV strains in Tibet was 97%, with foreign PPRV strains M protein amino acid homology ranges 96. 7% - 100%. M gene nucleotide phy- logenetic tree analysis showed that the Chinese PPRV 2013 ~ 2014 strains and the 3 strains of Tibet are not in the same small branch, and familiar with the India. In the process of this research, we inferred that China 2013 -2014 PPR epidemic outbreak in 2007 of non - continuation of Tibet, may be introduced into neighboring countries such as India.

关 键 词：小反刍兽疫 M基因 序列分析 

分 类 号：S855.3[农业科学—临床兽医学]