孤束核损毁术后艾灸足三里穴对胃黏膜损伤模型大鼠TNF-α、NO和HSP70表达的影响  

Effect of moxibustion at Zusanli point after damage to the nucleus tractus solitarius on expression of tumor necrosis factor-α,nitric oxide,and human heat shock protein 70 in rats with gastric mucosal injury

作　　者：李飞[1] 向丽婷 陈果[1] 向娟[1] 陈英[1] 彭亮[1] 李燕平 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省长沙市按摩骨科医院,湖南长沙410011

出　　处：《湖南中医杂志》2017年第5期146-149,共4页Hunan Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:80010397)

摘　　要：目的:观察孤束核捣毁术后艾灸"足三里"穴对胃黏膜损伤模型大鼠肿瘤坏死因子α(TNF-α)、血清一氧化氮(NO)与热休克蛋白(HSP70)表达的影响,并分析在艾灸保护胃黏膜损伤的神经通路中孤束核所起的作用。方法:将SD大鼠48只随机分为正常组(A组)、模型组(B组)、艾灸+模型组(C组)、艾灸+模型+孤束核损毁组(D组),每组12只,无水乙醇灌胃造成胃黏膜损伤,并用阿司匹林混悬液连续灌胃3d维持损伤(A组予0.9%氯化钠注射液灌胃),按Guth法计算胃黏膜损伤指数(UI),采用ELISA法检测胃黏膜细胞凋亡相关因子TNF-α、NO含量,并采用Western-blot法检测大鼠胃黏膜组织中保护蛋白HSP70的含量。结果:B组胃黏膜出现明显损伤,表明采用无水乙醇灌胃及阿司匹林悬浊液灌胃法造模成功(P<0.05);C、D组UI指数较B组均有不同程度的下降(P<0.01,P<0.05),D组UI指数较C组高(P<0.01)。C、D组胃黏膜组织中TNF-α含量较B组均有不同程度的下降,NO含量均有上升(P<0.05,P<0.01);D组胃黏膜组织中TNF-α含量较C组高,而NO含量较C组低(P<0.01)。胃黏膜保护蛋白HSP70的表达C、D组较B组均有明显上升(P<0.01),同时D组的表达较C组低(P<0.05)。结论:艾灸足三里穴对胃黏膜损伤有明显的保护性调节作用,而孤束核损毁后其保护效应受到明显抑制,证明对大鼠足三里穴进行温和灸后产生胃黏膜保护机制非常重要的传导中枢是孤束核。Objective：To investigate the effect of moxibustion at Zusanli point after damage to the nucleus tractus solitarius on the expression of tumor necrosis factor -α （ TNF -α） , serum nitric oxide （NO） , and human heat shock protein 70 （HSP70） in rats with gastric mucosal injury, as well as the role of moxibustion in protecting the nucleus tractus solitarius against gastric mucosal injury. Methods ： A total of 48 Sprague - Dawley rats were randomly divided into normal group （group A）, model group （group B ）, moxibustion ＋ model group （group C ）, and moxibustion ＋ model ＋ damage to the nucleus tractus solitarius group（group D） ,with 12 rats in each group. Gastric mucosal injury was induced by intragastric administration of anhydrous ethanol, and aspirin suspension was given by gavage for 3 days to maintain the injury（group A was given 0.9% sodium chloride injection by gavage）. The Guth method was used to calculate ulcer index（UI） ;ELISA was used to measure the content of apoptosis -related factors TNF -α and NO in gastric mucosal cells, and Western blot was used to measure the content of protective protein HSPT0 inthe gastric mucosa. Results：Group B had marked gastric mucosal injury, which suggested that the model was success- fully established by intragastric administration of anhydrous ethanol and aspirin suspension （P 〈0. 05）. Compared with group B, groups C and D had a significant reduction in UI （ P 〈 0. 01, P 〈 0.05 ） , and group D had a signifi- cantly higher UI than group C（P 〈0.01 ）. Compared with group B, groups C and D had a significant reduction in the content of TNF-α and a significant increase in the content of NO in the gastric mucosa（P 〈0.05 ,P 〈0. 01 ） ; compared with group C, group D had a significantly higher level of TNF - α and a significantly lower level of NO in the gastric mucosa（ P 〈 0.01 ）. Compared with group B, groups C and D had a significantincrease in the expression of HSP70（P 〈0.01）,and group D h

关 键 词：孤束核损毁 胃黏膜损伤 艾灸疗法 HSP70 TNF-Α NO 

分 类 号：R285.5[医药卫生—中药学]