球形电凝建立兔喉气管狭窄模型  

The laryngotracheal stenosis model induced by spherical electrocoagulation in rabbits

作　　者：黄汝施 郑宏良[1] 张才云[1] 陈世彩[1] 

机构地区：[1]第二军医大学附属长海医院,上海200433

出　　处：《解剖学杂志》2017年第3期303-306,共4页Chinese Journal of Anatomy

基　　金：军队重点课题(BWS14C019)

摘　　要：目的:利用球形电凝建立喉气管狭窄兔模型,探讨兔喉气管狭窄模型建立新方法。方法:新西兰大白兔分成实验组和对照组。实验组纵向切开气管第10~12环,用球形电凝(20 W)烧灼气管内壁5 s后闭合气管,对照组纵向切开气管后不做任何处理闭合气管。在术后第7、14、28天内镜观察气管瘢痕形成情况,之后处死动物,取出喉及上段气管,观察大体柄本,H-E及Masson染色后进行组织学观察。结果:内窥镜检查,对照组无瘢痕组织增生,实验组出现瘢痕组织增生,术后第7天与第14天的气管狭窄度范围10%~21%和21%~33%,中位数分别为16%和29%,术后第28天气管狭窄程度范围44%~62%。中位数55%。实验组气管狭窄度随着时间延长而逐渐增加,术后第28天气管狭窄度大于术后第7天。组终学观察显示,实验组动物气管狭窄部位黏膜下有少量炎性细胞浸润,成纤维细胞增生和大量胶原纤维增厚。结论:球形电凝可导致兔喉气管狭窄,可利用球形电凝建立喉气管狭窄,方法简单易行。Objective： To explore the feasibility of laryngotracheal stenosis model in rabbits induced by spherical eleetrocoagulation, Methods： Twenty-two New Zealand white rabbks were randomly divided into experimental group （n= 12） and control group （n=10）. Longitudinal incision was made along the 10th to 12th rings of the trachea, of which the inner surface was burned with spherical electrocoagulation （20 W） for 5 seconds in experimental animals. The tracheal scarring was endoscopieally observed on day 7,14 and 28. After being sacrificed at postoperative day 28, the larynx and the upper trachea tissue were harvested, and then submitted to H-E and Masson staining. Results： Scar formation was found in experimental trachea,not in control trachea during experimental course, The medlar degree of tracheal stenosis ranged from 16% on 7 d （10% to 21%）, 29% on 14 d （21% to 33%） to 55% on 28 d （44% to 62%）, among which statistical differences occurred between data from 7 d and 28 d. Conclusion： It is technically simple and reliable to model laryngotracheal stenosis in rabbits with spherical electrocoagulation.

关 键 词：喉气管狭窄 球形电凝 动物模型 兔 

分 类 号：R-332[医药卫生] R767