鸭疫里默氏杆菌假定溶血素相关蛋白基因Riean_0620的原核表达及溶血活性检测  

Prokaryotic expression and detection of hemolytic activity of hypothetical hemolysin-related protein gene Riean_0620 of Riemerella anatipestifer

作　　者：陶敏捷 姜盼[1] 孙冰清[1] 丁云竹 薛亚飞[1] 曹立松[2] 刘光清[1] 胡青海[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]安徽省望江县畜牧兽医局雷池兽医站,安徽望江246241

出　　处：《中国预防兽医学报》2017年第6期447-450,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31272590;31472224);上海市闵行领导人才专项资金

摘　　要：为研究鸭疫里默氏杆菌(RA)的假定溶血素相关蛋白Riean_0620的功能,本研究根据血清10型RAHXb2株Riean_0620基因序列设计引物,PCR扩增Riean_0620基因,并进行序列测定与分析。将Riean_0620基因克隆到原核表达载体p ET-43.1a进行诱导表达,采用亲和层析纯化获得重组蛋白r Riean_0620。溶血活性检测结果表明r Riean_0620具有裂解鸭红细胞的活性。本研究将有利于进一步研究Riean_0620蛋白的生物学特性以及RA的溶血机制。To investigate the biological function of hypothetical hemolysin-related protein Riean_0620 of R.anatipestifer, Riean_0620 gene from serotype 10 R.anatipestifer strain HXb2 was amplified by PCR and sequenced. Then, the gene ORF was inserted into the prokaryotic expression vector pET-43.1a. The expression of recombinant protein Riean_0620 was induced with IPTG and rRiean-0620 was purified with affinity chromatograph. The hemolytic activity assay showed the recombinant protein rRiean_0620 had the ability to lyse duck erythrocytes. The result of this study benefit to further study on biological functions of Riean_0620 protein and hemolysis mechanism of R.anatipestifer.

关 键 词：鸭疫里默氏杆菌 溶血素 原核表达 溶血活性检测 

分 类 号：S852.61[农业科学—基础兽医学]