甘薯MADS-box转录因子IbMADS11-Like的克隆及胁迫响应分析  被引量:3

Cloning and Expression Analysis of Stress Treatments of a MADS-box Transcription Factor in Sweet Potato

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作  者:谈传婷 宋炜涵 朱明库[1] 徐涛[1] 董婷婷[1] 李宗芸[1] 

机构地区:[1]江苏师范大学生命科学学院,徐州221116

出  处:《植物研究》2017年第4期587-595,627,共10页Bulletin of Botanical Research

基  金:国家自然科学基金(31501352);江苏省自然科学基金(BK20150229);大学生实践创新训练计划项目(201510320022;201510320022Z);江苏师范大学博士学位教师科研支持项目(14XLR012)~~

摘  要:MADS-box蛋白在植物生长发育及抗逆等过程中均发挥重要功能。本实验室根据甘薯近缘野生种I.trifida基因组序列,在甘薯栽培种徐薯22(Ipomoea batatas(L.)Xu22)中克隆到一个STMADS11亚家族MADS-box基因,命名为IbMADS11-Like。实时定量RT-PCR分析表明,IbMADS11-Like基因在甘薯根中大量表达,并且随着块根的形成和膨大表达量逐渐降低,表明该基因可能参与了甘薯块根的发育过程。胁迫处理分析表明,IbMADS11-Like基因的表达受干旱、盐和高温的诱导,而低温则抑制其表达。此外,IbMADS11-Like基因对ABA、IAA、ZT、BR、ACC、JA及GA等激素的处理也有不同程度的响应,暗示IbMADS11-Like基因可能参与了甘薯生长发育及胁迫的调控过程。这些结果为进一步分析IbMADS11-Like基因在甘薯块根发育和胁迫响应中的功能奠定了基础。MADS-box proteins play important functions in the development and stress response of plants. A STMADSll subfamily gene was cloned from Ipomoea batatas (L.) Xu22 according to I. trifida genome sequences, named IbMADS11-Like. By real-time RT-PCR IbMADS11-Like was expressed abundantly in roots, and a rapid declining trend was observed as storage root formation and development. The IbMADS11-Like might be involved in the storage root development of sweet potato. Stress treatment experiments showed that IbMADS11-Like was induced by drought, NaC1 and high temperature, while was reduced by low temperature. ABA, IAA, ZT, BR, ACC, JA and GA treatments also impacted the expression of IbMADS11-Like, indicating that IbMADS11-Like might play functions in the development and stress response in sweet potato. These results play a foundation for further study on the physiological function of IbMADS11-Like in the development and stress response of sweet potato.

关 键 词:MADS-box蛋白 IbMADS11-Like 转录因子 克隆 非生物胁迫 甘薯 

分 类 号:Q78[生物学—分子生物学] S531[农业科学—作物学]

 

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