响应面优化长白落叶松体胚形成早期培养条件的研究  

Response Surface Optimization of Culture Conditions for the Formation of Early Embryos in Larix olgensis

作　　者：宋跃[1] 李淑娟[1] 白晓明[1] 张含国[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2017年第4期613-620,共8页Bulletin of Botanical Research

基　　金：国家高技术研究发展计划("863"计划2013AA102704-04-03)~~

摘　　要：以长白落叶松胚性愈伤组织为材料,通过Box-Behnken Design设计试验,建立以体胚发生量为响应值的响应面模型,优化长白落叶松体胚形成早期的培养条件。结果表明:相对离子浓度(P=0.007)、肌醇浓度(P=0.000)、培养周期(P=0.000)、肌醇浓度与培养周期的交互作用(P=0.007)对长白落叶松体细胞胚胎发生量的影响极为显著。长白落叶松体胚形成早期的最适培养条件为:相对离子浓度26.767 7%BM、肌醇浓度10.454 5g·L^(-1)、培养周期12.656 6 d,预测体细胞胚胎的发生量为337.04个·g^(-1)。为便于实际操作,验证试验将培养条件调整为:离子浓度25%BM、肌醇浓度10 g·L^(-1)、培养周期13 d,体胚平均发生量为336.46个·g^(-1),与预测值336.29个·g^(-1)基本一致,体胚萌发率与生根率分别达100%和83.33±5.78%,与前期试验相比,长白落叶松体胚发生的数量和质量均有显著提高。With the larch embryogenic callus, through the Design Box-Behnken design test, we established a response surface model based on the somatic embryogenesis. The culture conditions of somatic embryo early maturation was optimized in Larix olgensis. The relative ion concentration （P = 0. 007 ）, the inositol concentration（ P = 0. 000）, the culture period （P = 0. 000） and the interaction between inositol content and culture period （P = 0. 007 ） effect on the number of somatic embryos of L. olgensis were extremely significant. Cultivation of Larix somatic embryo maturation was 337.04 ind · g-1 at the early stage of the most optimum conditions for relative ion content of 26.767 7% BM basic culture medium, inositol 10. 454 5 g · L-1 , culture time 12.656 6 d, and the number of somatic embryos average. In order to facilitate the practical operation, the culture condition was changed to the relative ion concentration of 25% BM, inositol 10 g · L-1, incubation period of 13 d, then the average number of somatic embryos was 336.46 ind .g-1, that was consistent with the predicted value 336.29 ind · g- 1. Compared with previous test, the quantity and quality of L. olgensis somatic embryos were increased significantly.

关 键 词：落叶松属 长白落叶松 响应面优化 体胚发生 胚胎形成早期 

分 类 号：S791.22[农业科学—林木遗传育种] Q943.1[农业科学—林学]