梅花鹿鹿茸多肽对小鼠ADSCs迁移、黏附活性及增殖的影响  

Effects of Sika Deer Velvet (Cervus nippon) Antler Polypeptides on Migration, Adhesion and Proliferation of ADSCs in Mouse (Mus musculus)

作　　者：张郑瑶[1] 王晓龙[1] 赵长伟[2] 王国祥[3] 

机构地区：[1]大连理工大学生命与医药学院,盘锦124000 [2]长春中医药大学,长春130000 [3]吉林大学第一医院肿瘤中心,长春130000

出　　处：《农业生物技术学报》2017年第7期1154-1161,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金青年基金项目(No.81503177);吉林省科技发展计划项目(白求恩专项)(No.20160101158JC)

摘　　要：梅花鹿(Cervus nippon)鹿茸32肽是从新鲜鹿茸中提取的一类多肽类物质,对骨髓间充质干细胞(mesenchymal stem cells,MSCs)有明显的促增殖和诱导分化活性,而脂肪间充质干细胞(adipose tissuederived stem cells,ADSCs)与骨髓间充质干细胞均来自于中胚层,在骨损伤修复和抗肿瘤的研究中备受关注。为了观察鹿茸多肽对小鼠(Mus musculus)ADSCs的增殖、迁移及黏附性的影响。细胞增殖实验(methyl thiazolyl tetrazolium assay,MTT)24 h后,实验组OD值与对照组无明显差别,48和72 h则明显高于对照组。细胞划痕实验结果表明,多肽浓度为1μg/m L时划痕宽度小于对照组,50及5μg/m L时大于对照组。胰蛋白酶化实验检测发现加入等剂量胰蛋白酶进行消化,随着多肽浓度的升高,细胞消化时间显著缩短,细胞黏附性逐渐降低。本研究表明,鹿茸多肽对ADSCs促增殖作用明显,呈现一定的剂量和时间依赖性,可以促进小鼠ADSCs增殖,降低细胞黏附力,低浓度多肽可促进细胞迁移,高浓度则抑制,提示鹿茸多肽可用于加速细胞增殖,降低其在特定区域的黏附性,同时为促进其向受损部位转移提供了可能,为骨肿瘤及损伤治疗提供了新思路。Velvet antler 32 polypeptide of sika deer （Cervus nippon）, which is a kind of peptide extracted from fresh antler, has obvious activity to promote proliferation and induce differentiation for mesenchymal stem cells （MSCs）. Both of adipose tissue-derived stem cells （ADSCs） and MSCs come from mesoderm, and they are high-profile in the research of bone injury repair and anti-tumor. In this research, the effect of velvet antler polypeptide on ADSCs proliferation, migration and adhesiveness have been observed. Methyl thiazolyl tetrazolium assay （MTT） showed that OD had no obvious difference between experiment group and control group after 24 h. But experiment group was obviously higher than control group after 48 and 72 h. Wound healing assay showed that the concentration of velvet antler polypeptide was 1 μg/mL, the scratch width was narrower than control group, and when the concentration was 50 or 5 Ixg/mL, the scratch width was bigger than control group. Trypsinization assay found that adding isotonic trypsin to digest, with the increase in peptide concentration, cell digestion time shortened significantly and cell adhesion decreased gradually. This research showed that antler polypeptide promoted proliferation of ADSCs, a certain dose and time-dependent could promote the proliferation of ADSCs in mouse （Mus musculus）, reducing cell adhesion and low concentrations of peptides could promote cell migration and high concentrations were inhibited. The results suggested that velvet peptides could be used to increase the number of seed cells and reduce its adhesion in specific areas. While providing the possibility of facilitating its transfer to the damaged site at the same time, which provides a new idea for bone tumor and injury treatment.

关 键 词：鹿茸多肽 脂肪间充质干细胞(ADSCs) 细胞增殖 迁移 黏附 

分 类 号：S185[农业科学—农业基础科学]