荜茇酰胺对人肺腺癌A549细胞的增殖及其辐射敏感性的影响  被引量:5

Effect of piplartine on proliferation activity and radio-sensitization of human pulmonary adenocarcinoma A549 cells line

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作  者:肖楠[1] 姚建新[1] 徐党辉[2] 李占峰[1] 潘志尧[3] 姚志峰[4] 

机构地区:[1]江苏联合职业技术学院南京卫生分院医学影像系,210038 [2]南京中医药大学第一附属医院放射科 [3]浙江大学医学院基础医学系 [4]南京医科大学第二附属医院放射治疗科

出  处:《山西医药杂志》2017年第11期1259-1262,共4页Shanxi Medical Journal

基  金:国家自然科学基金(30870741);江苏省自然科学基金(BK2007245)

摘  要:目的探讨荜茇酰胺对人肺腺癌A549细胞的增殖以及辐射增敏性的影响。方法体外培养人肺腺癌A549细胞,取对数生长期细胞进行实验。采用活细胞计数盒(CCK-8)法测定荜茇酰胺对A549细胞生长增殖的影响;流式细胞术观察细胞周期和凋亡。结果荜茇酰胺明显抑制A549细胞的增殖,引起细胞凋亡和细胞G_0/G_1期的阻滞。荜茇酰胺联合X射线照射能明显增加细胞的增殖抑制率和细胞凋亡。结论荜茇酰胺可明显抑制人肺腺癌细胞的生长增殖。低浓度的荜茇酰胺增加了人肺腺癌细胞的辐射敏感性,可能与其促进细胞的增殖抑制、诱导细胞凋亡、引起G_0/G_1期阻滞有关。[Abstract] Objective To investigate the effects of piplartine on cell proliferation and radio-sensitization of human pulmonary adenocarcinoma A549 cells line. Methods A549 cells were cultured in vitro and the ceils in log- arithmic growth phase were selected for experiments. Cell Counting Kit-8(CCK-8) assay was used to determine cell proliferation and radio-sensitization. Cell cycle and apoptosis were analyzed by flow cytometry(FCM). Results Piplartine inhibited the proliferation of A549 cells in a dose-and time- dependent pattern, induced cell apoptosis and caused G0/G1 arrest. Both the cell proliferation rates and apoptosis rates were increased by the combination treatment of piplartine and radiation. Conclusion These findings indicate that piplartine can inhibit cell growth ob- viously. Piplartine at non-cytotoxic concentrations can enhance the radio- sensitization of A549 cells,which seems to be associated with the enhancement of radiation-induced inhibition of cell proliferation and induction of apoptosis as well as Go/G1 arrest.

关 键 词:荜茇酰胺 肺腺癌A549细胞 辐射敏感性 增殖 细胞周期 凋亡 

分 类 号:R285[医药卫生—中药学]

 

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