刺芹侧耳多肽对染铅大鼠脑海马组织形态及海马组织中camk2a、erk2和homer1基因mRNA表达量的影响  

Effect of Pleurotus eryngii Polypeptides on Morphological Character and Expression of camk2a,erk2 and homer1 mRNA in Hippocampus of Lead-exposed Rats

作　　者：郝正祺 刘伟[1] 冯翠萍[1] 常明昌[1,2] 孟俊龙[1] 刘靖宇[1] 

机构地区：[1]山西农业大学食品科学与工程学院,山西太谷030801 [2]山西省食用菌工程技术研究中心,山西太谷030801

出　　处：《食用菌学报》2017年第2期49-54,共6页Acta Edulis Fungi

基　　金：山西省重点研发计划项目(201603D2011201);山西省科技攻关项目(20130311017-3);山西省煤基重点科技攻关项目(FT2014-03-01);黄土高原食用菌提质增效协同创新中心资助

摘　　要：为研究刺芹侧耳(Pleurotus eryngii)多肽对染铅大鼠脑组织损伤的影响,以雄性SD大鼠为研究对象,随机分为正常对照组(基础饲料,蒸馏水)、染铅模型组(基础饲料,0.2%醋酸铅水溶液)和多肽组(添加2%多肽的基础饲料,0.2%醋酸铅水溶液),饲养60d后,观察大鼠脑部海马组织形态变化,测定海马组织中铅含量、camk2a、erk2和homer1mRNA的表达量。结果表明:铅会导致海马组织的细胞数量减少、细胞层变薄、细胞间隙增大、camk2a、erk2和homer1mRNA表达量极显著降低;与染铅模型组相比,多肽组海马组织的铅含量显著降低、细胞数目增多、细胞核变大、细胞间隙缩小,同时海马组织中NMDAR和mGluR5信号通路相关基因camk2a、erk2和homer1mRNA的表达量显著或极显著升高,表明刺芹侧耳多肽可减轻铅对大鼠海马组织的损伤。To exlpore the effect of Pleurotus eryngii polypeptides on brain injury in lead acetate-exposed rats. Male SD rats were randomly divided intothree groups., control group （basal feed, distilled water）, leadexposed model group （basal feed, 0.2% aqueous solution of lead acetate） and polypeptide group （basal feed supplemented with 2% polypeptide, 0.2% aqueous solution of lead acetate）. After 60 days of polypeptide feeding, the results showed lead acetate could decrease number of cells in hippocampus, thickness of cell layer, expression levels of camk2a, erk2 and homer1 mRNA, and increase cell gap in lead acetate-exposed model control group. Compared with the lead-exposed model group, the polypeptide treatment could significantly induce decrease in lead content, increase in number of hippocampus cells, larger cell nucleus, and narrower cell gaps as well as significantly higher or extremely significantly higher expression levels of camk2a, erk2 and homerl mRNA relating to NMDAR and mGluR5 signaling pathways in hippocampus. These results indicated that P. eryngii could relieve the impairment of rat hippocampus induced by lead acetate.

关 键 词：刺芹侧耳多肽 N-甲基-D-天冬氨酸受体 MGLUR5 

分 类 号：R285.5[医药卫生—中药学]