两种无孢灵芝子实体醇提物的比较  

Comparison of Ethanolic Extracts from Fruit Bodies of Two Sporeless Cultivars of Ganoderma lingzhi

作　　者：钱丁寅 冯娜[2] 刘伟[1] 张劲松[2] 韩伟[1] 

机构地区：[1]华东理工大学药学院,上海200237 [2]上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,上海市农业遗传育种重点开放实验室,上海201403

出　　处：《食用菌学报》2017年第2期67-70,共4页Acta Edulis Fungi

基　　金：福建省教育厅菌草生态产业协同创新攻关课题(JCXTGG10)资助

摘　　要：以龙芝2号和鹿角灵芝两种无孢灵芝(Ganoderma lingzhi)子实体为原料,95%乙醇超声提取,对醇提物中的三萜进行了分析和体外抗肿瘤实验。结果表明,两种样品的醇提物得率分别为1.8%和3.7%,醇提物中三萜化合物含量分别为0.53%和1.16%;高效液相结果表明,鹿角灵芝中三萜的种类也多于龙芝2号。两种无孢灵芝的醇提物对肿瘤细胞K562和SW620的增殖均有一定的抑制作用,龙芝2号抑制肿瘤增殖的活性优于鹿角灵芝。Fruit bodies （5 g） of two sporeless cultivars of Ganoderma lingzhi, Longzhi NO. 2 （LZ） and Antler-shape G. lingzhi （LJ）, were immersed in 20 vols of 95% ethanol and extracted twice with ultrasound （600 W） for 30 min, the resultant ethanol extracts were then concentrated under vacuum respectively. Crude extract yields were 1.8% and 3.7% for LZ and LJ, triterpenoids content of the extracts were 0.53% and 1.16% correspondingly. HPLC （high performance liquid chromatography） profiles indicated that ethanol extracts of LJ contained more sorts of triterpenoids. Further experiment indicated that ethanol extracts of LZ showed stronger inhibition to the proliferations of K562 and SW620 cancer cells.

关 键 词：无孢灵芝菌株 醇提物 三萜 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]