高压均质法提取云芝胞内糖肽  

Extraction of Intracellular Polysaccharopeptide from Coriolus versicolor Mycelium Using Hot Water and High-Pressure Homogenization

作　　者：陈露[1] 陆震鸣[1] 龚劲松[1] 耿燕[1] 徐国华 张晓梅[1] 史劲松[1] 许正宏[1] 

机构地区：[1]江南大学药学院,江苏无锡214122 [2]江苏神华药业有限公司,江苏淮安211600

出　　处：《食用菌学报》2017年第2期79-82,共4页Acta Edulis Fungi

摘　　要：采用高压均质法提取云芝(Coriolus versicolor)胞内糖肽,与热水法进行比较。结果表明:高压均质提取的云芝胞内糖肽得率是热水提取的2倍,为(20.2±0.6)%;采用高压均质法提取的组分5相对百分含量最高,为48.07%;扫描电镜下观察发现,与热水提取相比,高压均质提取时云芝菌丝体破碎程度比较高。High-pressure homogenization （HPH） and hot water extraction （HWE）were used to extract intracellular polysaccharopeptide （IP） from Coriolus versicolor mycelia. Yields of IP extracted using HPH reached （20.2± 0.6）%, which were twice as high as those achieved using HWE. HPLC separated the IPs into six components, of which the component with a peak retention time of 26.46 mins made up 48.07% of the material extracted using HPH. Scanning electron microscopy revealed that HPH-treated mycelium had a more porous and ruptured structure compared to HWE-treated samples.

关 键 词：云芝 糖肽 高压均质 热水提取 高效液相色谱 

分 类 号：R284.2[医药卫生—中药学]