GRK2在帕罗西汀治疗大鼠Ⅰ型复杂区域疼痛综合征中的变化  

Study of G protein-coupled receptor kinase 2 in rat complex regional pain syndrome-typeⅠ treatment by paroxetine

作　　者：张弩[1] 方浩[1,2] 唐俊[3] 

机构地区：[1]复旦大学附属金山医院麻醉科,上海200540 [2]复旦大学附属中山医院麻醉科,上海200030 [3]复旦大学附属第五人民医院麻醉,上海200029

出　　处：《中国临床药学杂志》2017年第3期147-152,共6页Chinese Journal of Clinical Pharmacy

基　　金：复旦大学附属金山医院课题(编号2013-21)

摘　　要：目的研究帕罗西汀治疗大鼠上肢Ⅰ型复杂区域疼痛综合征(CRPS-Ⅰ)时颈上交感神经节(SCG)中G蛋白偶联受体激酶2(GRK2)的变化。方法采用单侧前肢缺血再灌注法制备SD大鼠前肢CRPS-Ⅰ模型。模型成功后随机分为帕罗西汀(P)组、溶媒(S)组、对照(C)组。模型建立后第3天,P组大鼠经尾静脉注射帕罗西汀10 mg·kg^(-1);S组大鼠注射3%乙醇生理盐水溶液1 mL·kg^(-1),均连续注射7 d;C组不予处理。于术前及术后1、2、7、14、21 d采用Von Fery纤毛测痛法与丙酮滴定法分别测量各组的机械痛阈值与冷刺激痛。采用免疫组化法定量颈上交感神经节内GRK2含量。结果 C组和S组大鼠实验侧缺血再灌注后的前趾机械痛阈值和冷痛觉阈值在术后2 d内均有下降,并持续至术后21 d。颈上交感神经节内GRK2含量在缺血再灌注后下降。对侧相应各项指标无明显改变。P组静脉给予帕罗西汀后,实验侧机械痛阈值和冷痛觉阈值的下降小于C组和S组,颈上交感神经节GRK2含量下降也较C组和S组弱。结论大鼠上肢CRPS-Ⅰ模型在机械刺激与冷刺激痛觉敏感增强的同时,SCG神经元内的GRK2明显下降,而帕罗西汀治疗后,机械刺激与冷刺激痛敏感减轻,颈上交感神经节内GRK2含量上升。AIM To pression in superior cervical I ） in rats. METHODS investigate the effects of paroxetine on G protein-coupled receptor kinase 2 （GRK2） exganglion （SCG） in forelimb model of complex regional pain syndrome type- I （CRPS- CRPS- I models were induced by ischemia reperfusion in the unilateral upper limbs of SD rats. All rats were assigned to 3 groups： group P received paroxetine （10 mg·kg^-1） every day for 7 days by injected through caudal veins after successfully modeling; group S were injected 3% ethanol saline solution every day for 7 days; group C were as control group, respectively. The 50% paw withdrawal thresholds （PWTs） to Von Fery mechanical stimuli, the cold allodynia to acetone titration and the GRK2 levels in the SCG were detected at the day before test, and the 1st day, the 2rid day, the 3rd day, the 14th day and the 21th day after injection. GRK2 levels were measured by using immunohistochemistry in superior cervical ganglion. RESULTS In the group C and the group S, the pain thresholds to mechanical stimuli and cold in the tested side upper limbs decreased from the 2nd day of ischemia reperfusion and lasted to 21 days. GRK2 levels in SCG of test side were decreased simultaneously. No changes were found in the contralateral limbs. In the group slightly compared to those in other groups. CONCLUSION P, both pain thresholds and GRK2 levels decreased In rat CRPS-I model, a decrease of GRK2 levels in SCG might be involved in the development and maintenance of allodynia to mechanic and cold stimulation. Paroxetine might alleviate allodynia through upregulation of GRK2 protein

关 键 词：G蛋白偶联受体激酶2 Ⅰ型复杂区域疼痛综合征 颈上交感神经节 机械和冷刺激痛觉过敏 帕罗西汀 

分 类 号：R965[医药卫生—药理学]