白藜芦醇抑制软骨肉瘤细胞SW1353炎症反应的实验研究  

Effects of resveratrol on inhibiting inflammatory response in SW1353 chondrocytes

作　　者：于晓璐[1] 刘旭丹[1] 姜梦琪[1] 徐小磊[1,2] 何健宜 顾海伦[3] 刘莉[1] 

机构地区：[1]中国医科大学公共卫生学院营养与食品卫生学教研室,辽宁沈阳110122 [2]北华大学公共卫生学院营养与食品卫生学教研室,吉林吉林132012 [3]中国医科大学附属盛京医院骨科,辽宁沈阳110004

出　　处：《东南大学学报（医学版）》2017年第3期337-342,共6页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金资助项目(81372971)

摘　　要：目的:探讨白藜芦醇(RES)对白细胞介素1β(IL-1β)处理的人软骨肉瘤细胞SW1353的效应及其可能机制。方法:采用CCK-8法测定不同浓度IL-1β及RES对SW1353细胞增殖活力的影响。ELISA法检测细胞培养基上清基质金属蛋白酶13(MMP-13)的水平。RT-PCR法检测Toll样受体4(TLR4)、TIR结构域接头分子(TRIF)、髓样分化蛋白88(My D88)mRNA表达。结果:IL-1β处理对SW1353细胞的增殖活力无明显抑制作用(P>0.05)。RES浓度为6.25、12.5μmol·L^(-1)时能促进细胞增殖(P<0.05)。RES浓度在50μmol·L^(-1)时对细胞增殖无明显作用(P>0.05),而RES浓度为200μmol·L^(-1)时则显著抑制细胞增殖(P<0.05)。IL-1β和RES联合处理与单纯RES处理比较,能够降低细胞增殖活力(P<0.05)。相比对照组,IL-1β处理组培养基上清中MMP-13分泌水平及TLR4、TRIF、My D88 mRNA表达均明显增加(P<0.01),而RES则能够显著抑制IL-1β诱导的MMP-13的分泌水平,并降低TLR4、TRIF、My D88 mRNA的表达(P<0.01)。结论:RES可以通过抑制TLR4信号通路对体外培养的SW1353细胞发挥抗骨性关节炎效应。Objective： To investigate the effect and possible mechanisms of resveratrol on inflammatory response in SW1353 cells induced by IL-1β.Methods： Proliferation ability of SW1353 cell in different concentrations of IL-1β and resveratrol was detected by the CCK-8 assay.The levels of MMP-13 in cell culture supernatants were measured by ELISA.The expressions of TLR4, TRIF and MyD88 mRNA were examined by RT-PCR.Results： CCK-8 assay showed that IL-1β had no obvious effect on SW1353 cells proliferation（P〉0.05）.At 6.25 and 12.5 μmol·L-1, resveratrol promoted cell proliferation（P＆lt;0.05）.There was no discernable inhibition effect on cells with resveratrol at 50 μmol·L-1（P〉0.05）.However, resveratrol had significant inhibition effect on cell proliferation when the concentration was 200 μmol·L-1（P＆lt;0.05）.In addition,compared with the resveratrol group, cell proliferation ability was reduced in the combination group of IL-1β and resveratrol（P＆lt;0.05）.The levels of MMP-13 and the expression of TLR4,TRIF and MyD88 mRNA were significantly up-regulated in the presence of IL-1β compared with the control group（P＆lt;0.01）,while resveratrol treatment could significantly down-regulate them（P＆lt;0.01）.Conclusion： Resveratrol may exert anti-inflammatory effect on SW1353 cells via the inhibition of TLR4 signaling pathway.

关 键 词：软骨内瘤细胞 白藜芦醇 TOLL样受体4 TIR结构域接头分子 髓样分化蛋白88 

分 类 号：R285.5[医药卫生—中药学]