血清miR-598-3p与CA153联合检测在乳腺癌诊断中的意义  

Combined determination of serum miR-598-3p and CA153 in the diagnosis of breast carcinoma

作　　者：苏谦[1] 陈文举[1] 王攀[1] 李招云[1] 

机构地区：[1]台州市中心医院检验科,浙江台州318000

出　　处：《检验医学》2017年第6期457-460,共4页Laboratory Medicine

基　　金：浙江省科技计划项目(2012C37037);台州市科技计划项目(2011ky0701);台州市医学会科研研究项目(TZSYXH15-27)

摘　　要：目的探讨血清微小RNA-598-3p(miR-598-3p)检测及miR-598-3p与糖类抗原153(CA153)联合检测在乳腺癌诊断中的意义。方法收集2013年1月—2014年9月在台州市中心医院确诊的100例乳腺癌患者、45例乳腺良性疾病患者及40名健康对照者血清标本,应用实时荧光定量聚合酶链反应(PCR)检测血清miR-598-3p的表达水平,应用化学发光法检测血清CA153水平,应用受试者工作特征(ROC)曲线分析血清miR-598-3p、CA153及二者联合检测对乳腺癌诊断的敏感性和特异性。结果乳腺癌患者血清miR-598-3p的表达水平下调,CA153水平升高,与健康对照组比较,差异均有统计学意义(P<0.01)。miR-598-3p的表达量与乳腺癌TNM分期无相关性(P>0.05)。ROC曲线分析结果显示,miR-598-3p诊断乳腺癌的敏感性和特异性分别为95.0%、85.0%;CA153诊断乳腺癌的敏感性和特异性分别为66.0%、65.0%。二者联合检测诊断乳腺癌的敏感性和特异性分别为96.0%、70.0%。结论 miR-598-3p对乳腺癌的诊断具有良好的敏感性和特异性,联合CA153检测可以提高对乳腺癌的诊断效能。Objective To investigate the combined determination of serum microRNA-598-3p（miR-598-3p） and carbohydrate antigen 153（CA153）in the diagnosis of breast carcinoma.Methods A total of 100 breast carcinoma patients,45 benign breast disease controls and 40 healthy controls in Taizhou Central Hospital from January 2013 to September 2014. The level of serum miR-598-3p was determined by real-time fluorescence quantitation polymerase chain reaction（PCR）. The level of serum CA153 was determined by chemiluminescence. Receiver operating characteristic（ROC） curve was used to evaluate the sensitivities and specificities of serum miR-598-3p and CA153 single and combined determinations for diagnosing breast carcinoma.Results Compared to healthy control group,serum miR-598-3p was down-regulated,and serum CA153 was up-regulated in breast carcinoma group（P〈0.01）. There was no correlation between serum miR-598-3p level and different TNM stages of breast carcinoma（P〉0.05）. ROC curve showed that the sensitivities of serum miR-598-3p and CA153 were 95.0% and 66.0%,and the specificities were 85.0% and 65.0%,respectively. The sensitivity and specificity of the combined determination of serum miR-598-3p and CA153 were 96.0% and 70.0%.Conclusions MiR-598-3p shows a good sensitivity and specificity for diagnosing breast carcinoma,and the combined determination of serum miR-598-3p and CA153 could improve diagnostic efficiency.

关 键 词：微小RNA-598-3p 实时荧光定量聚合酶链反应 乳腺癌 

分 类 号：R446.62[医药卫生—诊断学]