表观健康人群血清脂蛋白相关磷脂酶A_2参考区间的建立  

Establishment on the reference intervals of serum lipoprotein-associated phospholipase A_2 in apparently healthy adults

作　　者：丰睿捷 胡晓晨[1] 赵莉芳[2] 张海晨[1] 宋云霄[1] 

机构地区：[1]复旦大学附属中山医院徐汇医院检验科,上海200031 [2]复旦大学附属中山医院徐汇医院心内科,上海200031

出　　处：《检验医学》2017年第6期495-499,共5页Laboratory Medicine

摘　　要：目的建立上海地区表观健康人群血清脂蛋白相关磷脂酶A_2(Lp-PLA_2)的参考区间。方法检测800名健康体检者(男、女性各400名)血清Lp-PLA_2活性,根据美国临床实验室标准化协会(CLSI)C28-A3文件的相关要求,以百分位数法确定Lp-PLA_2的95%参考区间[第2.5百分位数(P2.5)~第97.5百分位数(P97.5),可信区间为90%]。结果表观健康人群血清Lp-PLA_2活性呈正态分布。男性Lp-PLA_2活性为(479±126)U/L,女性为(433±118)U/L,二者间差异有统计学意义(t=5.311,P<0.01)。男性血清LpPLA_2活性保持恒定,女性血清Lp-PLA_2活性随年龄的增加而呈升高趋势,各年龄段Lp-PLA_2活性比较差异有统计学意义(F=2.017,P<0.05)。进一步分析显示<50岁的各年龄组之间和≥50岁的各年龄组之间Lp-PLA_2活性差异均无统计学意义(P>0.05),因此将女性各年龄组合并为18~49岁组和50~89岁组。由此得出上海地区表观健康人群血清Lp-PLA_2的参考区间男性为219~694 U/L,女性18~49岁为204~651 U/L、50~89岁为217~686 U/L。结论初步建立了上海地区表观健康人群血清Lp-PLA_2的参考区间,为临床应用Lp-PLA_2作为心血管疾病风险评估指标提供依据。Objective To establish the reference intervals of serum lipoprotein-associated phospholipase A2 （Lp-PLA2）in apparently healthy adults.Methods The activities of serum Lp-PLA2 in 800 apparently healthy adults（400 males and 400 females） were determined,and the 95% reference intervals [the 2.5th percentile （P2.5）-the 97.5th percentile（P97.5）,90% confidence interval] were determined,according to the Clinical and Laboratory Standards Institute（CLSI）C28-A3.Results Serum Lp-PLA2 activities showed a normal distribution in apparently healthy adults. The reference intervals of serum Lp-PLA2 activities were（479＆#177;126） U/L in males and （433＆#177;118） U/L in females with statistical significance（t=5.311,P〈0.01）. Serum Lp-PLA2 activities were stable in males,while those in females increased with age,and there was statistical significance for age（F=2.017, P〈0.05）. There was no statistical significance for serum Lp-PLA2 activities in females between 〈50 years old and ≥50 years old（P〉0.05）,and thus females could be classified into 18-49 years old group and 50-89 years old group. Therefore,the reference intervals of serum Lp-PLA2 activities in apparently healthy adults were 219-694 U/L for males,204-651 U/L for 18-49 years old females and 217-686 U/L for 50-89 years old females.Conclusions The reference intervals of serum Lp-PLA2 activities in apparently healthy adults have been established initially,which provide a reference for the clinical application of serum Lp-PLA2 as an indicator for the assessment of cardiovascular risk.

关 键 词：脂蛋白相关磷脂酶A2 表观健康成人 参考区间 Lipoprotein-associated PHOSPHOLIPASE A2 

分 类 号：Q556[生物学—生物化学]