肠炎相关肠癌小鼠模型及急性肠炎小鼠模型特征研究  

Investigation of characters of acute colitis mice model and colitis-associated cancer mice model

作　　者：阿来依·买提卡比力 阿不来提·阿合买提[2] 木塔力甫·艾买提[1] 阿仙姑·哈斯木[3] 

机构地区：[1]新疆医科大学中心实验室,乌鲁木齐830011 [2]新疆医科大学基础医学院机能中心,乌鲁木齐830011 [3]新疆医科大学基础医学院病理解剖学教研室,乌鲁木齐830011

出　　处：《新疆医科大学学报》2017年第8期1069-1073,共5页Journal of Xinjiang Medical University

基　　金：新疆医科大学2013年度科研创新基金(XJC201303)

摘　　要：目的探讨肠炎相关肠癌小鼠模型及急性肠炎小鼠模型特征。方法雄性C57BL/6小鼠(实验组)给予5%葡聚糖硫酸钠(Dextran sulfate sodium,DSS)连续饮用5d,5d后换为饮用蒸馏水至21d,构建急性肠炎小鼠模型,对照组21d内均饮用蒸馏水。肠炎相关肠癌小鼠模型的构建方法:雄性C57BL/6小鼠(实验组)腹腔注射氧化偶氮甲烷(Azoxymethane AOM)1次,1w后予3%DSS连续饮用5d,随后12d饮用蒸馏水,DSS/蒸馏水循环共3次。对照组腹腔注射同体积生理盐水,饮用蒸馏水。采用HE染色观察病理学特征变化,RT-PCR法检测急性肠炎炎症因子表达特征。结果急性肠炎模型发生炎症过程中小鼠体质量降低,相关炎症因子白细胞介素6(IL-6)、白细胞介素10(IL-10)、白细胞介素17(IL-17)表达变化趋势与肠黏膜结构变化过程相符,在炎症剧烈阶段(6~9d)时表达均上调,而随着肠黏膜结构的自我修复,表达趋于正常。AOM/DSS肠炎相关肠癌模型可在3个DSS循环后形成肉眼可见腺瘤。结论本研究为利用此模型进行后期的治疗炎症性肠病(IBD)药物筛查提供可靠数据支持。Objective To investigate characters of acute colitis mice model and colitis-associated cancer （CAC） mice model. Methods Builded up acute colitis mice model by 5 % DSS （Dextran sulfate sodium） o- ral administration for 5 days on C57BL/6 （test group） mice with distilled water till to 21 days. And set up colitis-associated cancer mice model by intraperitoneal injection of AOM （Azoxymethane） once followed by three cycles （5 days/cycle） of 3% DSS oral administration to develop CAC tumors. HE staining way was used to check the pathological characters and RT-PCR technique was to check characters of cytokines ex-pressions. Results Weight loss occurred during DSS-colitis process, and a high production of IL-10, IL-6 and IL-17 were found on 6 - 9 d after the treatment, meanwhile the epithelial status was destroyed most seriously then started repair itself in the 17 days or 21 days. Adenocarcinoma were visible in gut under mi-croscope in CAC mice model. Conclusion This investigation may be useful as a convenient tool to validate candidate therapeutic drugs for IBD.

关 键 词：DSS模型 AOM/DSS模型 肠黏膜结构 腺瘤 

分 类 号：R29[医药卫生—民族医学]