多项免疫学指标在青霉素过敏死亡鉴定中的价值  

The value of multiple immunological indexes in the identification of anaphylactic death by penicillin

作　　者：解勋 谢海[2] 卢延旭[1] 王飞虎[1] 

机构地区：[1]中国刑事警察学院法医学系,辽宁沈阳110035 [2]佛山市公安局禅城分局,广东佛山528011

出　　处：《中国法医学杂志》2017年第3期261-265,共5页Chinese Journal of Forensic Medicine

基　　金：辽宁省教育科学"十三五"规划立项课题(JG16DB469)

摘　　要：目的考察类胰蛋白酶、类糜蛋白酶、IL-4和IL-10在青霉素过敏死亡豚鼠死后0~48h的水平或表达。方法采用青霉噻唑蛋白致敏和激发,豚鼠死后0~48 h提取血液或组织,采用免疫组织化学法和ELISA法分别检测类胰蛋白酶与类糜蛋白酶组织表达和IL-4、IL-10水平。结果与对照组相比较,实验组肺和气管中类胰蛋白酶和类糜蛋白酶的表达增强,血清、肺、气管中的IL-4、IL-10水平增高(P<0.05)。结论类胰蛋白酶、类糜蛋白酶、IL-4和IL-10在青霉素过敏死亡鉴定中具有重要价值。Objective To investigate the expression level of tryptase, chymase, IL-4 and IL-10 in guinea pigs died from anaphylactic death caused by penicillin allergy within 48 hours. Methods Guinea pigs were sensitized and elicited by penicilloyl-protein, the blood and tissues were extracted within 48 hours after the death. The expression of tryptase and chymase in tracheas and lungs were detected by the ways of immunohistochemistry, IL-4 and IL-10 levels in serum, tracheas and lungs were detected by ELISA. Results Compared with the control group, the expression of tryptase and chymase has enhanced in lungs and tracheas, the level of IL-4 and IL-10 increased in serum, lungs and tracheas in experimental groups（P〈0.05）. Conclusion Tryptase, chymase, IL-4 and IL-10 have significant value in the identification of the deaths caused by penicillin allergy.

关 键 词：法医病理学 青霉素过敏死亡 免疫学方法 肥大细胞蛋白酶 白介素 

分 类 号：D919.4[医药卫生—法医学]