溴敌隆及其代谢物-苄叉丙酮在犬体内的死后分布  

The postmortem distribution of bromadiolone and its metabolite-benzylideneacetone in poisoned dogs

作　　者：赵丹玭 杨泽国 尉志文[1] 傅善林 刘良[4] 贠克明[1] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]河津县公安局,山西河津044300 [3]悉尼科技大学法庭科学中心 [4]华中科技大学同济医学院法医学系,武汉430030

出　　处：《中国法医学杂志》2017年第3期294-297,共4页Chinese Journal of Forensic Medicine

基　　金：国家科技支撑计划项目(2007BAK26B05;2012 BAK02B02-2);国家自然科学基金(81172906);山西省回国留学人员科研资助项目(2014-032)

摘　　要：目的研究溴敌隆及其代谢物-苄叉丙酮在中毒致死犬体内死后分布规律,为溴敌隆中毒检材的采取提供实验依据。方法分别经口给予犬2倍和4倍LD_(50)溴敌隆,待其死亡后迅速解剖取材,气相色谱-质谱联用法测定心血、外周血、尿、胆汁、心、肝、脾、肺、肾、脑、左下肢肌、膀胱、胃、胃内容、胰等脏器和体液中溴敌隆和代谢物-苄叉丙酮的含量。结果犬经2倍和4倍LD_(50)溴敌隆灌胃染毒后3d开始出现出血症状,(178.40±20.94)h后死亡。溴敌隆和代谢物-苄叉丙酮在各组织脏器及体液中的死后分布为:溴敌隆2LD_(50)组溴敌隆:胆汁>尿、肝、心、肾>心血、外周血、脾、肺等;苄叉丙酮:胆汁、尿、心血、外周血、肺、胃内容中含量高于其他脏器。溴敌隆4LD_(50)组溴敌隆:胆汁、尿>肝、外周血>心血、胃内容物等脏器。苄叉丙酮:胆汁、尿、肺浓度高于其他脏器。结论溴敌隆及其代谢物-苄叉丙酮在中毒致死犬体内死后分布不均匀,溴敌隆在胆汁、尿、肝脏、心血和外周血含量较高,代谢物-苄叉丙酮在胆汁、尿、肺较高。胆汁、尿、肝脏、心血、外周血可作为疑似溴敌隆中毒毒物分析的检材。Objective To study the postmortem distribution of Bromadiolone and its metabolite-Benzylideneacetone in dogs and provide an experimental evidence for the sampling of Bromadiolone poisoning cases. Methods The dogs were given 2LD50 and 4LD50 Bromadiolone by intragastric administration. Anatomy was conducted immediately after death and samples of body fluids and viscera（heart blood; peripheral blood, bile, urine, heart, liver, spleen, lung, kidney, brain, urinary bladder, left leg muscle, stomach, stomach contents, pancreas） were collected and detected after the dogs poisoning death. The Bromadiolon and its metabolite-Benzylideneacetone contents in samples were analyzed by GC/MS. Results Hemorrhagic symptoms came out at 3d after Bromadiolone delivery and deaths occurred at（178.40±20.94）h after intoxication. The postmortem distribution of Bromadiolon and its metabolite-Benzylideneacetone in dogs was as following： 2LD50 Bromadiolon： bile〉urine, liver, heart, kidney〉heart blood, peripheral blood, spleen, lung and so on. Benzylideneacetone： the content in bile, urine, heart blood, peripheral blood, lung, stomach contents are higher. 4LD50 Bromadiolon： bile, urine〉liver, peripheral blood〉heart blood, stomach contents and others. Benzylideneacetone： contents in bile, urine and lung are higher. Conclusion The postmortem distribution of Bromadiolon and its metaboliteBenzylideneacetone in dogs is uneven, contents in bile, urine, liver, heart blood and peripheral blood are higher, which are suggested for forensic toxicological analysis in Bromadiolon poisonig case.

关 键 词：法医毒物分析 死后分布 溴敌隆 代谢物-苄叉丙酮 GC-MS/MS 

分 类 号：D919.4[医药卫生—法医学]