中脑腹侧被盖区多巴胺神经元在全身麻醉中的作用研究  

Research on effect of midbrain VTA dopaminergic neurons in general anesthesia

作　　者：刘程曦 谭丹丹[1] 李佳[1] 张益[1] 

机构地区：[1]遵义医学院麻醉系,贵州遵义563000

出　　处：《重庆医学》2017年第17期2320-2322,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81560237)

摘　　要：目的探讨中脑腹侧被盖区(VTA)多巴胺神经元在全身麻醉诱导和苏醒中的作用。方法成年健康雄性SD大鼠40只,分为毁损组(n=20)和对照组(n=20),毁损组在双侧VTA给予特异性多巴胺神经元毁损药6-羟多巴胺(6-OHDA)减少多巴胺神经元,对照组在双侧中脑腹侧被盖区给予等量生理盐水。待手术后两周,观察在全身麻醉下,大鼠翻正反射消失时间(LORR)和恢复时间(RORR)。结果与对照组相比,在丙泊酚麻醉下可显著缩短大鼠LORR(P<0.05),且明显延长大鼠RORR(P<0.05)。在异氟醚麻醉下毁损组与对照组相比,大鼠LORR,差异无统计学意义(P>0.05),但大鼠RORR延长(P<0.05)。结论 VTA多巴胺神经元在不同的全身麻醉药的诱导和苏醒发挥着有不同作用。Objective To investigate the effect of dopaminergic neurons of midbrain ventral tegmental area（VTA） in general anesthesia. Methods Forty adult healthy male SD rats were randomly divided into lesion group （n= 20） and control group （n=20）. The lesion group was given the bilateral infusion of specific dopaminergic neuron injury agent 6-OHDA in midbrain lateral VTA,while the control group received the same volume of normal saline at the same areas. The time of loss of righting reflex CLORR）loss and recovery of r^ghting ret＇lex（RORR）at postoperative 2 week were observed in each group. Results Compared with the control group,the LORR time in the lesion group was shortened and the RORR time was significantly prolonged under propo- fol-induced anesthesia （P〈0.05）. However,the LORR time under the isoflurane anesthesia had no statistically significant difference between the two groups（P〉0. 05）, while the RORR time in the lesion group was increased（P〈0.05）. Conclusion Dopaminergic neurons in midbrain VTA might play different roles in the induction and recovery of different general anesthetics.

关 键 词：丙泊酚麻醉 异氟醚麻醉 中脑腹侧被盖区 多巴胺神经元 翻正发射 

分 类 号：R614.2[医药卫生—麻醉学]