miRNA-1和miRNA-21在脂联素后处理保护大鼠缺血-再灌注心肌中的变化  

The role of miRNA-I and miRNA-21 in adiponectin postconditioning on myocardial ischemia reperfusion injury in rats

作　　者：赵林静[1] 崔柳苏[1] 张金盈[2] 王永玲[1] 殷俊磊[1] 

机构地区：[1]新乡医学院基础医学院,河南453003 [2]郑州大学第一附属医院心血管内科,河南450000

出　　处：《重庆医学》2017年第A01期16-18,共3页Chongqing medicine

基　　金：新乡医学院2016年度博士科研启动基金资助（XYBSKYZZ201634）.

摘　　要：目的探讨miRNAhl和miRNA-21在脂联素（ADP）后处理对大鼠心肌缺血-再灌注损伤（MIRI）保护过程中的变化和意义。方法将SD大鼠随机分为假手术组（Sham组）、MIRI组和ADP后处理组（ADP组），每组10只。用Elisa检测各组大鼠血浆心肌肌钙蛋白I（cTnI）水平，用Westernblot检测各组大鼠心肌磷酸化／蛋白激酶B（p-Akt）的表达，用realtimeRT-PCR检测各组大鼠心肌中miRNA_1和miRNA-21的表达。结果与Sham组比较，MIRI组大鼠血浆cTnI水平和心肌miRNA-l表达明显升高，心肌p-Akt和miRNA一21表达明显降低，差异均有统计学意义（P〈0．05）；与MIRI组比较，ADP组大鼠血浆cTnI水平和心肌miRNA-1表达明显降低，心肌P-Akt和miRNA-21表达明显升高，差异均有统计学意义（P〈O．05）。心肌miRNA-1表达与血浆cTnI水平呈正相关（r=0．703；P〈0．05），与心肌P—Akt表达呈负相关（r=-0796；P〈0．05）；心肌miRNA-21表达与血浆cTnI水平呈负相关（r=-0．727；Pd0．05），与心肌p—Akt表达呈正相关（r=0.886；P〈0．05）。结论miRNA_l是大鼠MIRI及ADP后处理过程中的负性调节因子，miRNA-21是MIRI及ADP后处理过程中的正性调节因子，p-Akt蛋白可能参与了上述调节过程。Objective To observe the changes of two micro-RNA（miRNA-1 and miRNA-21） during adiponectin（ADP） postcon- ditioning against myocardial ischemia reperfusion injury（MIRI） in rats, to study the possible implications of the changes above. Methods SD rats were divided randomly into 3 groups（n=10）：（1）Group Sham（group in sham operated）;（2）Group MIRI（group in myocardial ischemia reperfusion injury） ;（3）Group ADP（group in ADP postconditioning） were subjected to injection of ADP be- fore reperfusion. The ti.ter of cardiac troponin I（cTnI） in plasma and the the expressions of p-Akt protein in myocardial tissue were observed,the relative expressions of miRNA-1 and miRNA-21 in myocardial tissue in each group were determined by RT-PCR re- spectively. Results Compared with Group Sham,cTnI in plasma and miRNA-1 in myocardial tissue in Group MIRI increased（P〈0.05）, p-Akt and miRNA-21 in myocardial tissue declined （P〈0.05） ;compared with Group MIRI, cTnI in plasma and miRNA-1 in myocardial tissue in Group ADP decreased （P^0.05）, p-Akt and miRNA-21 in Group ADP increased（P〈0.05）, miRNA-1 corre- lated positively with cTnI in plasma and negatively with p-Akt in myocardial tissue（P〈0.05） ;miRNA-21 correlated negatively with cTnI in plasma and positively with p-Akt in myocardial tissue（P〈0.05）. Conclusion In the process of MIRI and ADP post- conditioning against MIRI in rats,miRNA-1 is a negative regulatory factor while miRNA-21 is a positive regulatory factor. The reg- ulation above is perherps related to p-Akt.

关 键 词：脂联素 缺血后处理 心肌 缺血一再灌注损伤 miRNA-1 MIRNA-21 磷酸化蛋白激酶B 

分 类 号：R654.2[医药卫生—外科学]