Embryoid Induction and Cell Suspension Culture of Premna microphylla Turca  

作　　者：Hua CHENG Zhichao LI Linling LI Shuiyuan CHENG 

机构地区：[1]College of Life Science,Huanggang Normal University [2]Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratory

出　　处：《Agricultural Biotechnology》2017年第3期6-9,15,共5页农业生物技术（英文版）

基　　金：Supported by Nature Science Foundation of Hubei Province(2008CDZ085)

摘　　要：In order to find suspension culture conditions suitable for Premna microphylla Turea, with the leaves of experimented P. microphylla as a material, em- bryogenic callus induction, subculture, dispersion of embryogenic callus and suspension culture were performed. The results showed that the calli induced from leaves were different. The medium most suitable for induction of loose embryogenic callus for suspension culture was MS ＋ 0.4 mg/L 2,4-D ＋ 0.4 mg/L NAA ＋ 3% sucrose ＋0.8% agar. A better cell line could be obtained with cellulase and pectinase for dispersion of P. microphyUa callus. The medium most suitable for sus- pension culture was MS ＋0.4 mg/L 2,4-D ＋0.8 mg/L KT ＋3% sucrose. In this medium, the fresh weight of the suspensian-cultured cells increased by 6.46 times per 7 d. Dark culture was more suitable for cell proliferation. The average contents of pectin and protein were, respectively, 6.57% and 0.12% in the sus- pended ceils of P. microphylla, which had been cultured for 3 weeks under the optimum culture conditions.In order to find suspension culture conditions suitable for Premna microphylla Turea, with the leaves of experimented P. microphylla as a material, em- bryogenic callus induction, subculture, dispersion of embryogenic callus and suspension culture were performed. The results showed that the calli induced from leaves were different. The medium most suitable for induction of loose embryogenic callus for suspension culture was MS ＋ 0.4 mg/L 2,4-D ＋ 0.4 mg/L NAA ＋ 3% sucrose ＋0.8% agar. A better cell line could be obtained with cellulase and pectinase for dispersion of P. microphyUa callus. The medium most suitable for sus- pension culture was MS ＋0.4 mg/L 2,4-D ＋0.8 mg/L KT ＋3% sucrose. In this medium, the fresh weight of the suspensian-cultured cells increased by 6.46 times per 7 d. Dark culture was more suitable for cell proliferation. The average contents of pectin and protein were, respectively, 6.57% and 0.12% in the sus- pended ceils of P. microphylla, which had been cultured for 3 weeks under the optimum culture conditions.

关 键 词：Premna microphylla Turcz CALLUS Suspension culture 

分 类 号：S567.19[农业科学—中草药栽培]