表没食子儿茶素没食子酸酯对稳定表达淀粉样前体蛋白的SH-SY5Y细胞抗炎抗凋亡作用研究  

A Study on the Anti-inflammatory and Anti-apoptosis Effect of EGCG on APP-expressing SH-SY5Y Cells

作　　者：王晶[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110032

出　　处：《成都医学院学报》2017年第3期254-259,共6页Journal of Chengdu Medical College

摘　　要：目的探讨表没食子儿茶素没食子酸酯(epigallocatechingallate,EGCG)对稳定表达淀粉样前体蛋白(amyloid precursor protein,APP)的SH-SY5Y细胞抗炎抗凋亡的保护作用。方法将SH-SY5Y细胞分为对照组、模型组(稳定表达APP的细胞)和EGCG组(APP+10、20、30μmol/L的EGCG)。免疫荧光细胞化学法观察APP在各组细胞中表达;MTT法检测细胞存活能力;TUNEL法检测细胞凋亡情况;RT-PCR法检测Bax、Bcl-2和Caspase-3表达;ELISA法测定IL-6和TNF-α浓度。结果模型组中,APP高表达;与模型组比较,给予20μmol/L EGCG的EGCG组明显提高SH-SY5Y细胞的存活率,减少细胞凋亡数目,下调Bax/Bcl-2比例及Caspase-3mRNA表达,减少IL-6和TNF-α浓度。结论 EGCG对稳定表达APP的SH-SY5Y细胞有一定保护作用,可能是通过抗炎以及减少细胞凋亡发挥治疗作用。Objective To investigate the anti-inflammatory and anti-apoptosis effect of EGCG on human SHSY5 Ycells expressing amyloid precursor protein（APP）stably.Methods The SH-SY5 Y cells were randomly divided into three groups including the control group,model group（SH-SY5 Ycells expressing APP stably）,and EGCG group（APP-expressing cells treated with 10,20 and 30μmol/L EGCG respectively）.The expression of APP was confirmed by the immunofluoresence staining;the cell viability was assessed by MTT;the number of cell apoptosis was assessed by TUNEL;the mRNA expression of Bax,Bcl-2and Caspase-3 were determined by RTPCR;the concentration of IL-6and TNF-αwas assessed by ELISA.Results The APP expression was higher in the model group.Compared with the model group,The administration of 20μmol/L EGCG promoted the survival rate of SH-SY5 Ycells,reduced the number of apoptotic cells,downregulated the radio of Bax and Bcl-2and the mRNA expression of Caspase-3,and decreased the concentration of IL-6and TNF-α.Conclusion EGCG has protective effect on the APP-expressing SH-SY5 Ycells and the therapeutic effect may be achieved by anti-inflammation and anti-apoptosis.

关 键 词：EGCG APP 阿尔茨海默病 SH-SY5Y细胞 细胞凋亡 

分 类 号：R284[医药卫生—中药学]