一种阿霉素/NO供体光敏纳米复合物的合成与性质研究  

Synthesis and properties study of Dox/NO donor nanocomposites

作　　者：殷姝君 王晓静[2,3] 王兵[1,2,3] 梅桂雪 孙捷[2,3] 

机构地区：[1]济南大学山东省医学科学院医学与生命科学学院,山东济南250200 [2]山东省医学科学院药物研究所,山东济南250062 [3]国家卫生部生物技术药物重点实验室山东省罕少见病重点实验室,山东济南250062

出　　处：《化学研究》2017年第3期371-377,共7页Chemical Research

基　　金：山东省自然科学基金(ZR2015YL041)

摘　　要：以硫化铜纳米晶(CuS-NCs)为核心,聚N-异丙基丙烯酰胺接枝壳聚糖(PNIPAM-g-CS)微粒为壳合成一种新型光敏纳米复合材料.在温度的调节下,N-异丙基丙烯酰胺(NIPAM)包覆CuS纳米晶,并接枝壳聚糖(CS),合成CuS杂PNIPAM-g-CS纳米复合材料.CuS在近红外光(980 nm)照射下具有光热效应,导致纳米复合物中PNIPAM-g-CS微粒受热体积收缩.负载阿霉素,这种纳米复合物就可作为光热诱导释放阿霉素的多功能纳米载体.再负载NO光敏供体(RBS),就可制备出阿霉素/RBS双负载的CuS杂PNIPAM-g-CS纳米载体.在可见光(365 nm)照射下,RBS光解释放NO.近红外光和可见光分别触发纳米载体释放阿霉素和NO,加上CuS纳米晶的光热效应,这种纳米载体可实现光触发双药物释放协同光热化疗杀伤肿瘤细胞.In this paper,we described the synthesis of a novel light-sensitive nanocomposites that consisted of CuS nanocrystal as the core and poly(N-isopropylacrylamide)-graft-chitosan(PNIPAM-gCS) microgel as the shell. The CuS@ PNIPAM-g-CS nanocomposites were synthesized by temperaturetunable copolymerization of NIPAM and CS in the presence of CuS nanocrystals. Due to the photothermal effect of CuS under NIR light(980 nm) irradiation,the nanocomposites presented photothermalsensitive volume shrinkage of PNIPAM-g-CS microgel. After loading of doxorubicin(Dox),the nanocomposites were utilized as versatile nanocarriers for photothermal-induced release of Dox. Nitric oxide(NO) photodonors(RBS) were then loaded into nanocomposites to fabricate Dox/RBS dual-loading CuS@ PNIPAM-g-CS nanocarriers. Upon visible light(365 nm) irradiation,the RBS could release NO due to the photolysis. Experimental results implied that NIR and visible light respectively triggered release of Dox and NO from nanocarriers. Together with photothermal effect of CuS,the nanocarriers realized light-triggered release of dual-drugs and synergistic chem-photothermal therapy to cancer cells.

关 键 词：CUS 纳米复合材料 药物释放 光热疗法 

分 类 号：O62[理学—有机化学]