角蛋白组氨酸金属结合体的制备及其对SOD酶的模拟  

Preparation of keratin combining with histidine and metal ions complexes and its simulation of SOD

作　　者：何玉凤[1,2,3] 陈静 闫桂芳[1,2,3] 李晓晓 钱立忠[1,2,3] 王荣民 

机构地区：[1]生态环境相关高分子材料教育部重点实验室,甘肃省高分子材料重点实验室,西北师范大学化学化工学院,甘肃兰州730070

出　　处：《化学研究与应用》2017年第6期780-785,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(21263024,21364012)资助

摘　　要：本文将羽毛角蛋白(FK)与组氨酸(His)复合,再与金属离子M(Cu^(II)、Zn^(II)、Ni^(II)、Mn^(II)络合成功制得了角蛋白组氨酸金属结合体FK-HisM_x(M=Cu、Zn、Ni、Mn)。采用紫外光谱(UV-Vis)、红外光谱(IR)、圆二色谱(CD)、热重分析(TGA)、扫描电镜(SEM)等手段对FK-HisM_x(M=Cu、Zn、Ni、Mn)的结构进行了表征。用核黄素/DL-甲硫氨酸-NBT还原法考察了FK-HisM_x对超氧阴离子自由基(O_2·^-)的清除作用,研究了其清除机理。结果发现:FK-His M_x均具有清除O_2·^-的能力。其中,FK-HisCu_x具有较高的O_2·^-清除活性,FK-HisCu_(60)的O_2·^-清除活性最好,其抗O_2·^-的EC_(50)值为0.0115μmol·L^(-1),对Cu,ZnSOD的模拟度高达356.5%。The water-soluble biopolymer,feather keratin combining with histidine metal ions complexes（ FK-His Mx）,were prepared by the soluble feather keratin（ FK） conjugating with histidine and metal ion（ Cu^II,Zn^II,Ni^II,Mn^II）. The structures of FK-His Mxwere characterized by UV-Vis,IR,TGA,CD and SEM.The superoxide anion radical（ O2^·- scavenging activity of FK-His Mxwas investigated by NBT photochemical reduction and the effect mechanism of scavenging O2^·-as also explored. The results show that the conjugates with different metal ions had antioxidant activities. After combining with the biopolymer FK,the Cu conjugate（ FKHis Mx） shows the most excellent scavenging ability of superoxide anion radical（ O2^·-,EC_（50） was 0. 0115μmol/L and its simulated degree reached 356. 5%for nature Cu,ZnSOD.

关 键 词：SOD模拟物 O2^·-清除活性 生物高分子金属结合体 氨基酸金属络合物 羽毛角蛋白 

分 类 号：O629.33[理学—有机化学]