HPLC法同时测定不同产地龙血竭中3种黄酮类成分  

Simultaneous Determination of Three kinds of Flavonoids in Resina Draconis from Different regions by HPLC

作　　者：李竣[1] 朱功俊 陈素[2] 刘向明[2] 李芸芳[1] 杨光忠[1] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]中南民族大学生物医学工程学院,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2017年第2期45-48,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(81403186);湖北省高等学校优秀中青年科技创新团队计划资助项目(T201220);中央高校基本科研业务费专项(3212015CZW15029;3212015YCZW15095);中南民族大学实验室开放与技改资助项目(KF2016005;JG2016004)

摘　　要：为建立HPLC测定不同产地龙血竭中3种黄酮类成分的方法,选择了广西、云南产9个批次的龙血竭,测定了其中龙血素A、龙血素B、剑叶龙血素B的含量.采用Thermo BDS Hypersil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-1%冰醋酸,梯度洗脱,流速1.0 m L·min^(-1),柱温40℃,检测波长278 nm.3个药效成分在45min内达到基线分离,线性关系良好.9个批次龙血竭中龙血素A、龙血素B、剑叶龙血素B的平均含量分别为:0.47%,0.96%,0.44%;平均加样回收率分别为:99.6%,103.2%,100.5%;RSD分别为:1.20%,0.65%,1.30%.该实验方法操作简便,结果准确,重复性和稳定性良好,可为龙血竭的质量控制提供参考.To establish a method for the determination of loureirin A, loureirin B and cochinchinenin B in Resina Draconis from Guangxi and Yunnan Province in China, HPLC testing was performed on a Thermo BDS Hypersil C18 column （4.6 mm×250 mm） with mobile phases of acetonitrile-1% acetic acid solution in a gradient mode, with a flow rate of 1.0 mL·min-1, column temperature of 40℃, and detection wavelength of 278 nm.The three components were baseline separated in 45 mins and showed good linear relationship.The average content of loureirin A, loureirin B and cochinchinenin B in the nine batches of Resina Draconis were 0.47%, 0.96%, 0.44%, and the average recover rates were 99.6% （RSD=1.20%）, 103.2% （RSD=0.65%）, 100.5% （RSD=1.30%）, respectively.This method was convenient, accurate and reliable, which could provide reference for the quality control of Resina Draconis.

关 键 词：龙血竭 龙血素A 龙血素B 剑叶龙血素B HPLC法 

分 类 号：TQ460.72[医药卫生—药物分析学] R927.2[化学工程—制药化工]