HPLC法测定鱼肝油软膏中全反式维生素A醋酸酯的含量  

Determination of all-trans Vitamin A acetate in cod liver oil ointment by HPLC

作　　者：蔡乐[1] 白林[1] 范金钊[1] 任韡[1] 黄晓舞[1] 

机构地区：[1]解放军总医院药学部,北京100853

出　　处：《中国药物应用与监测》2017年第3期143-145,共3页Chinese Journal of Drug Application and Monitoring

基　　金：解放军总医院临床扶持基金(2012FC-CXYY-4008)

摘　　要：目的:建立HPLC法测定鱼肝油软膏中全反式维生素A醋酸酯的含量。方法:采用超声提取方法处理鱼肝油软膏,用HPLC法进行含量测定。色谱柱为Platisil ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相:甲醇-水(98:2),流速:1.0 m L·min(-1),检测波长:328 nm,柱温:30℃。结果:维生素A在0.587 9~7.348 5 U·m L^(-1)范围内线性关系良好(r=0.999 9);样品平均回收率为99.78%(RSD=1.57%)。结论:本方法简便、准确、重复性好,可用于鱼肝油软膏中全反式维生素A醋酸酯的含量测定。Objective： To establish the HPLC method for determination of all-trans Vitamin A acetate in cod liver oil ointment. Methods： The Vitamin A acetate in ointment was extracted by ultrasonic method, and the content of all-trans Vitamin A acetate was determinated by HPLC method. HPLC was carried out on a column of Platisil ODS C18 （250 mm × 4.6 mm, 5 μm） with mobile phase consisting of methanol-water （98 ： 2）. The fow rate was 1.0 mL·min^-1. The detection wavelength was set at 328 nm. The column temperature was 30 ℃. Results： Vitamin A showed a good linear relationship in the range of 0.587 9 – 7.348 5 U·mL^-1 （r = 0.999 9）. The average recovery was 99.78% （RSD = 1.57%）. Conclusion： The method is simple, accurate and repeatable, which is suitable for the content determination of all-trans Vitamin A acetate in cod liver oil ointment.

关 键 词：鱼肝油软膏 维生素A醋酸酯 高效液相色谱法 含量测定 

分 类 号：O657.72[理学—分析化学] R927.2[理学—化学]