二尖瓣退行性变瓣膜组织中Sox9表达的研究  

The study of Sox9 expression in degenerative mitral valve disease

作　　者：刘良[1] 刘晓红[1] 徐志云[1] 

机构地区：[1]第二军医大学附属长海医院胸心外科,上海200433

出　　处：《国际心血管病杂志》2017年第2期119-122,共4页International Journal of Cardiovascular Disease

基　　金：国家自然科学基金(81370335)

摘　　要：目的:探讨Sox9在二尖瓣退行性变(DMVD)与正常二尖瓣瓣膜组织中的表达差异。方法:收集11例DMVD患者(DMVD组)二尖瓣瓣膜与3例因其他疾病死亡患者(对照组)的正常二尖瓣瓣膜,行免疫组织化学染色,根据Sox9阳性细胞比例及阳性细胞染色强度,采用二次记分法判读,0~3分为Sox9低表达,4~9分为Sox9高表达,对两组Sox9表达情况进行统计学分析。结果:Sox9表达定位于细胞核,在对照组二尖瓣瓣膜内皮细胞、海绵层间质细胞内散在表达;Sox9在DMVD组二尖瓣瓣膜主要表达于间质细胞,各层均有分布,在海绵层呈团簇状分布。对照组二尖瓣瓣膜Sox9阳性细胞比例及染色强度均较低,其中Sox9低表达3例,高表达0例,综合评分1~3分;DMVD组二尖瓣瓣膜内Sox9低表达2例,高表达9例,综合评分3~9分;Sox9在DMVD组和对照组二尖瓣瓣膜中的表达差异有统计学意义(P=0.027)。结论:Sox9在DMVD二尖瓣瓣膜中高表达,可能参与二尖瓣瓣膜退行性变病变过程。Objective:To investigate the expression of Sox9 in mitral valves of degenerative mitral valve disease(DMVD)and normal mitral valves. Methods:Mitral valves from 11 DMVD patients(DMVD group)and 3 patients who died from other diseases(control group)were collected and stained by immunohistochemistry.According to the proportion of Sox9 positive cells and the staining intensity of positive cells,two scoring systems were used.The scores from 0 to 3 were regarded as Sox9 low expression,while 4 to 9 regarded as Sox9 high expression.Then statistical analysis was done according to the expression results of two groups. Results:Sox9 expressed in the nucleus,which had scattered expression in endothelial cells and spongiosa interstitial cells of normal valves in control group.While in the lesion valves of DMWD group,Sox9 mainly expressed in the interstitial cells of each layer,and was cluster-like distribution in the spongiosa.The proportion of Sox9 positive cells and the intensity of staining were lower in control group,including 3 cases with Sox9 low expression and 0 cases with high expression,whose overall scores were between 1 to 3;while Sox9 was higher expressed in DMVD group,with low expression in2 cases and high expression in9 cases,whose overall scores were between3 to 9.Sox9 expression was significantly different between DMVD group and control group(P =0.027). Conclusion:Sox9 is highly expressed in the mitral valves of patients with DMVD and may be involved in the process of mitral degeneration.

关 键 词：二尖瓣退行性变 SOX9 免疫组织化学 

分 类 号：R542.51[医药卫生—心血管疾病]