基于CAPS标记的甜瓜种子相关性状QTL分析  

QTL Analysis on Related Seed Traits in Melon Based on CAPS Markers

作　　者：叶伟震 刘识[1,2] 马鸿艳[1,2] 刘宏宇[1,2] 李桂英[1,2] 栾非时[1,2] 

机构地区：[1]东北农业大学农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江哈尔滨150030 [2]东北农业大学园艺园林学院,黑龙江哈尔滨150030

出　　处：《北方园艺》2017年第12期119-128,共10页Northern Horticulture

摘　　要：以种子相关性状差异显著的甜瓜品系MR-1和M1-15为试材,构建BC1P1回交群体,利用2个亲本材料全基因组重测序数据发掘SNP位点并将其转化为CAPS标记,构建遗传连锁图谱并对种子相关性状进行QTL分析。结果表明:获得了一张包含197个CAPS标记、12个连锁群的甜瓜遗传连锁图谱。检测到与种子相关性状紧密连锁的QTL位点6个,分布在1、2、5、8号染色体上,可解释9.86%~13.52%的表型变异率,贡献率>10%的QTL位点5个。Two melon inbred lines MR-I and M1-15 with a significant difference in the seed traits were used as rruaterials,to make a cross for BC1P1 genetic population. The SNP loci was developed and converted into CAPS markers based on the whole genome re-sequencing data of the two parental materials to construct genetic linkage map and QTL analysis of seed related traits. The results showed that a genetic linkage map containing 197 CAPS markers was obtained with 12 linkage groups and 6 QTLs for the seed related traits were detected and distribution in chromosome 1,2,5 and 8. The value range the value of five QTLs were more than 10%. Keywords. melon; seed traits; SNP; CAPS; genetic linkage of phenotypic variation was from 9. 86% to 13. 52% and the value of five QTLs were more than 10%.

关 键 词：甜瓜 种子性状 SNP CAPS 遗传连锁图谱 QTL 

分 类 号：S652.603.6[农业科学—果树学]