341例少弱精子症及无精子症患者细胞遗传学分析  

Cytogenetics abnormalities in 341 males with oligozoospermia and azoospermia

作　　者：徐清华[1] 吴小华 王琳琳[1] 张敏[1] 李冬秀[1] 许鹏宇[1] 马振勇[2] 

机构地区：[1]白求恩国际和平医院妇产科生殖中心 [2]白求恩国际和平医院256临床部,石家庄050082

出　　处：《中国优生与遗传杂志》2017年第6期62-64,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究少弱精子症及无精子症患者细胞染色体异常及染色体多态性的发生情况。方法对341例男性不孕不育患者(弱精子症165例,少精子症34例,重度少精子症44例,少弱精子症79例,无精子症19例)的外周血淋巴细胞进行培养、制片及G显带染色体核型分析。结果染色体异常27例,异常率7.9%。常染色体异常为16例,异常率为4.7%;性染色体异常11例,异常率为3.2%。染色体异常率弱精子症占3.0%,少精子症占5.8%,重度少精子症占18.1%,少弱精子症占6.3%,无精子症占36.8%。染色体多态性变异61例,占17.8%。结论男性不孕不育患者染色体异常和多态性发生率明显较高,对该类患者有必要常规做染色体检查,尤其在实施辅助生殖技术之前,以避免将遗传缺陷传递给下一代。Objective： To investige the chromosome aberrations and polymorphic variants in infertile men with oligozoospermia and azoospermia. Methods： The 341 infertile men including 165 asthenospermic cases, 34 oligospermic cases, 44 severe oligozoospermia cases, 79 oligoasthenospermic cases and 19 azoospermic cases were studied. Karyoptying was performed on peripheral blood lymphocytes by using the Giemsa trypsin banding （GTG） banding technique. Results： Chromosome analysis of 341 infertile males showed major chromosome abnormalities in 7.9%, with 4.7% in autosomal chromosome abnormalities and 3.2% in sex chromosome abnormalities. The incidence of major chromosome abnormalities in asthenospermic males were 3.0%, oligospermic males were 5.8%, severe oligozoospermia were 18.1%, oligoasthenospermic males were 6.3% and azoospermic males were 36.8%. Chromosomal polymorphic variants were identifed to be 17.8%. Conclusion： The overall high prevalence of chromosomal abnormalities in infertile males suggests that the conventional chromosomal analysis is an important investigative tool for male infertility, especially prior to use of any assisted reproductive techniques.

关 键 词：男性不育症 无精子症 少精子症 染色体畸变 染色体多态性 

分 类 号：R440[医药卫生—诊断学] R698.2[医药卫生—临床医学]