青龙衣含药血清抑制胃癌SGC-7901细胞生长和诱导凋亡  

Serum Containing Juglans mandshurica Pericarp Inhibits Proliferation and Induces Apoptosis of Gastric Carcinoma SGC-7901 Cell

作　　者：高启龙 朱亚楠 石变 马旭辉 耿良 张新峰 宁寒冰[2] 刘怀民 

机构地区：[1]郑州大学附属肿瘤医院,郑州450008 [2]郑州大学第一附属医院,郑州450000

出　　处：《中国实验方剂学杂志》2017年第13期111-115,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省医学科技攻关计划项目(122300410079);郑州市金水区科技攻关项目(金科201433;第二批39号)

摘　　要：目的:探讨青龙衣含药血清对胃癌SGC-7901细胞增殖及凋亡的影响,并探讨其作用机制。方法:Wistar大鼠随机分为空白组、青龙衣低剂量(25 g·kg^(-1)·d^(-1)),中剂量(50 g·kg^(-1)·d^(-1)),高剂量(100 g·kg^(-1)·d^(-1))组及顺铂(5 g·kg·d^(-1))组,连续灌胃给药7 d,空白组灌胃给予等体积生理盐水;末次给药2 h后,颈动脉采血,制备青龙衣含药血清;分别采用噻唑蓝(MTT)比色法、流式细胞术、烟酸己可碱(Hoechst)染色、实时荧光定量聚合酶链式反应(Real-time PCR)及蛋白免疫印迹法(Western blot)检测青龙衣含药血清对SGC-7901细胞增殖、凋亡率、凋亡指数、凋亡相关基因及凋亡信号通路蛋白表达的影响。结果:与空白组比较,青龙衣含药血清对SGC-7901细胞的增殖具有明显抑制作用(P<0.05),青龙衣含药血清处理48 h后的早期、晚期凋亡率,凋亡指数及促凋亡基因Bcl-2相关X蛋白(Bax),Bcl-2相关K蛋白(Bak)mRNA表达均升高,抗凋亡基因B淋巴细胞瘤-2(Bcl-2)mRNA表达降低(P<0.05);青龙衣含药血清组β-链蛋白(β-catenin),细胞周期蛋白D1(Cyclin D1)及原癌基因(C-myc)蛋白表达均明显降低(P<0.05)。结论:青龙衣含药血清对胃癌SGC-7901细胞的增殖具有抑制作用,该作用可能与诱导凋亡及抑制分泌型糖蛋白(Wnt)/β-catenin通路的活化有关。Objective： To explore the effects of serum containing Juglans mandshurica （SCJM） pericarp on proliferation and apoptosis of gastric carcinoma SGC-7901 cells. Method： Wistar rats were randomly divided into blank control group, low （25 g.kg-1.d-1） , medium （50 g.kg-1.d-1） and high （100 g.kg-1.d-1） dose J. mandshurica treatment groups and cisplatin group （5 g.kg-1.d-1 ）. The rats in treatment groups received lavage administration for 7 d, and the rats in blank control group received the equivalent volume of normal saline. 2 h after the last treatment, SCJM was prepared by carotid blood serum; cell proliferation, apoptosis rates, apoptosis index, levels of apoptosis related gene and protein expression were evaluated by methyhhiazolyldiphenyl-tetrazolium bromide （MTT） colorimetry, flow cytometry, Hoechst staining, Real-time PCR and Western blot respectively. Result： SCJM could inhibit the proliferation of SGC-7901 cells （P 〈 0.05） as compared with blank group, and 48 h after treatment by SCJM, early-and late-apoptosis rates, apoptosis index and mRNA levels of Bax and Bak were increased, but mRNA level of B-cell lymphoma-2 （Bcl-2） was decreased （P 〈0.05） ; the protein levels ofβ- catenin, Cyclin D1 and C-myc in SCJM-treatment groups were lower than those of control group （P 〈 0.05 ）. Conclusion： SCJM had inhibitory effect on proliferation of gastric carcinoma SGC-7901 cells, and the effect may be related to the induction of apoptosis and inhibition of Wnt/β-catenin pathway activation.

关 键 词：青龙衣含药血清 胃癌 细胞增殖 凋亡 分泌型糖蛋白(Wnt)/β-链蛋白(β-catenin) 

分 类 号：R285.5[医药卫生—中药学]