海珠益肝加味方对免疫性肝损伤小鼠细胞因子的影响  

Effect of Haizhu Yigan Jiawei formula on Cell Factor in Immunity Hepatic Injury mice

作　　者：徐建良[1,2] 张云城[2] 盛国光[1,2] 黄育华[1,2] XU Jian-liang ZHANG Yun-cheng HUANG Yu-hua et al(Department of Liver Diseases, Hubei Hospital of TCM Wuhan Hubei, 430061 China)

机构地区：[1]湖北省中医院肝病科,湖北武汉430061 [2]湖北省中医药研究院

出　　处：《中西医结合肝病杂志》2017年第3期168-169,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

摘　　要：目的:观察海珠益肝加味方对刀豆球蛋白A(Con A)诱导建立的免疫性肝损伤小鼠细胞因子IL-4、IL-6、INF-γ、TNF-a的影响,探讨其作用机制。方法:将40只昆明小鼠随机分为正常组、模型组、醋酸强的松组、海珠益肝加味方组,采用尾静脉注射Con A建立免疫性肝损伤小鼠模型,眼球取血,检测小鼠血清IL-4、IL-6、IFN-γ、TNF-α的水平。结果:与正常组比较,模型组小鼠外周血IL-4、IL-6水平明显降低(P<0.01),IFN-γ、TNF-α水平显著升高(P<0.05);与模型组比较,醋酸强的松组、海珠益肝加味方组小鼠外周血IL-4、IL-6的水平明显升高(P<0.01),IFN-γ、TNF-α水平显著降低(P<0.05)。结论:海珠益肝加味方能升高免疫性肝损伤小鼠细胞因子IL-6、IL-4的分泌水平,降低IFN-γ、TNF-α的水平,恢复Th1/Th2细胞之间的平衡,具有保护肝细胞的作用,可能是其作用机制之一。Objective： To observe the effect of Haizhu Yigan Jiawei formula on cell factor of IL-4, IL-6, INF-γ, TNF-α in immunity hepat- ic injury mice. Methods： Forty kun ming mice were randomly divided into normal control group, model group, prednisone group, HZYGJWF group, by Con A tail vein injections made immune liver injury model, after taking blood through eye sockets,measuring the level of IL-4, IL- 6, INF-γ, TNF-α. Results： Compared with normal control group, the level of IL-4 and IL-6 in Model group mice were decreased （ P 〈 0. 01 ） , the level of IFN-γ and TNF-α in model group mice were increased（ P 〈 0.05 ） ; Compared with model group, the level of IL- 14 and IL- 6 in prednisone group, HZYGJWF group mice were obviously heightened （P 〈 0.01 ） , the level of IFN-γ and TNF-α in model group mice were obviousiy reduced（ P 〈 0.05 ）. Conclusion： Haizhu Yigan Jiawei formula can heighten the level of IL-4 and IL-6 in Immunity Hepatic injury Mice, reducethe level of IFN-γ and TNF-α,and can adjust the balance between Th1/Th2 ,can protect liver, may be one of the mecha- nisms

关 键 词：海珠益肝加味方 刀豆蛋白A 免疫性肝损伤 细胞因子 

分 类 号：R285.5[医药卫生—中药学]